PUBLICATION

In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish

Authors
Liu, M., Schmitner, N., Sandrian, M.G., Zabihian, B., Hermann, B., Salvenmoser, W., Meyer, D., and Drexler, W.
ID
ZDB-PUB-131121-15
Date
2013
Source
Biomedical Optics Express   4(10): 1846-1855 (Journal)
Registered Authors
Meyer, Dirk, Schmitner, Nicole
Keywords
none
MeSH Terms
none
PubMed
24156048 Full text @ Biomed. Opt. Express
Abstract

For the first time the far red fluorescent protein (FP) E2-Crimson genetically expressed in the exocrine pancreas of adult zebrafish has been non-invasively mapped in 3D in vivo using photoacoustic tomography (PAT). The distribution of E2-Crimson in the exocrine pancreas acquired by PAT was confirmed using epifluorescence imaging and histology, with optical coherence tomography (OCT) providing complementary structural information. This work demonstrates the depth advantage of PAT to resolve FP in an animal model and establishes the value of E2-Crimson for PAT studies of transgenic models, laying the foundation for future longitudinal studies of the zebrafish as a model of diseases affecting inner organs.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes