p35 promotes the differentiation of amacrine cell subtype in the zebrafish retina under the regulation of egr1
- Authors
- Zhang, L., Bonilla, S., Zhang, Y., and Leung, Y.F.
- ID
- ZDB-PUB-131113-5
- Date
- 2014
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 243(2): 315-23 (Journal)
- Registered Authors
- Bonilla, Sylvia, Leung, Yuk Fai, Zhang, Liyun
- Keywords
- p35, cdk5r1b, zebrafish, retinal development, cdk5, egr1, amacrine cells, smarca4
- MeSH Terms
-
- Amacrine Cells/physiology*
- Animals
- Cell Differentiation/genetics
- Cell Differentiation/physiology*
- DNA Primers/genetics
- Early Growth Response Protein 1/metabolism*
- Gene Expression Regulation, Developmental/genetics*
- Gene Knockdown Techniques
- Image Processing, Computer-Assisted
- Immunohistochemistry
- In Situ Hybridization
- Microscopy, Fluorescence
- Morpholinos/genetics
- Nerve Tissue Proteins/metabolism*
- Parvalbumins/metabolism
- Retina/embryology*
- Retina/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- PubMed
- 24115595 Full text @ Dev. Dyn.
Background: Early growth response 1 (egr1) is a transcription factor (TF) for controlling the differentiation of Parvalbumin (Parv) -expressing amacrine cells (ACs) in zebrafish. However, the downstream factors of this process have not been identified. The purpose of this study was to investigate the role of p35, a neuronal-specific activator of cyclin-dependent kinase 5 (Cdk5) and a known in vitro target of egr1, in the differentiation of these ACs. Results: In the p35-knockdown retinas, Parv+ but not islet1+ ACs were specifically reduced. This phenotype was highly similar to that in the Egr1-knockdown retinas. Furthermore, p35 expression was reduced in the Egr1-knockdown retinas, particularly in the AC region; while egr1 was only modestly reduced in this region in the p35-knockdown retinas. Conclusions: p35 likely acts downstream of egr1 to control the differentiation of Parv+ ACs.