ZFIN ID: ZDB-PUB-131024-7
Blue Light-Mediated Manipulation of Transcription Factor Activity In Vivo
Masuda, S., Nakatani, Y., Ren, S., and Tanaka, M.
Date: 2013
Source: ACS Chemical Biology   8(12): 2649-53 (Journal)
Registered Authors: Tanaka, Mikiko
Keywords: none
MeSH Terms:
  • Animals
  • Bacterial Proteins/chemistry
  • Bacterial Proteins/genetics
  • Bacterial Proteins/metabolism*
  • Embryo, Nonmammalian
  • Fetal Proteins
  • Gene Expression Regulation, Developmental/radiation effects*
  • Light
  • Mutant Chimeric Proteins/chemistry
  • Mutant Chimeric Proteins/genetics
  • Mutant Chimeric Proteins/metabolism*
  • Photoreceptors, Microbial/chemistry
  • Photoreceptors, Microbial/metabolism
  • Protein Binding
  • Protein Multimerization
  • Protein Structure, Tertiary
  • Synechocystis/chemistry
  • Synechocystis/metabolism
  • T-Box Domain Proteins/chemistry
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Transcription, Genetic/radiation effects*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 24063403 Full text @ ACS Chem. Biol.

We developed a novel technique for manipulating the activity of transcription factors with blue light (termed “PICCORO”) using the bacterial BLUF-type photoreceptor protein PixD. The chimeric dominant-negative T-box transcription factor No Tail formed heterologous complexes with a PixD decamer in a light-dependent manner, and these complexes affected transcription repressor activity. When applied to zebrafish embryos, PICCORO permitted regulation of the activity of the mutant No Tail in response to 472-nm light provided by a light-emitting diode.