PUBLICATION
Blue Light-Mediated Manipulation of Transcription Factor Activity In Vivo
- Authors
- Masuda, S., Nakatani, Y., Ren, S., and Tanaka, M.
- ID
- ZDB-PUB-131024-7
- Date
- 2013
- Source
- ACS Chemical Biology 8(12): 2649-53 (Journal)
- Registered Authors
- Tanaka, Mikiko
- Keywords
- none
- MeSH Terms
-
- Animals
- Bacterial Proteins/chemistry
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism*
- Embryo, Nonmammalian
- Fetal Proteins
- Gene Expression Regulation, Developmental/radiation effects*
- Light
- Mutant Chimeric Proteins/chemistry
- Mutant Chimeric Proteins/genetics
- Mutant Chimeric Proteins/metabolism*
- Photoreceptors, Microbial/chemistry
- Photoreceptors, Microbial/metabolism
- Protein Binding
- Protein Multimerization
- Protein Structure, Tertiary
- Synechocystis/chemistry
- Synechocystis/metabolism
- T-Box Domain Proteins/chemistry
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism*
- Transcription, Genetic/radiation effects*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 24063403 Full text @ ACS Chem. Biol.
Citation
Masuda, S., Nakatani, Y., Ren, S., and Tanaka, M. (2013) Blue Light-Mediated Manipulation of Transcription Factor Activity In Vivo. ACS Chemical Biology. 8(12):2649-53.
Abstract
We developed a novel technique for manipulating the activity of transcription factors with blue light (termed “PICCORO”) using the bacterial BLUF-type photoreceptor protein PixD. The chimeric dominant-negative T-box transcription factor No Tail formed heterologous complexes with a PixD decamer in a light-dependent manner, and these complexes affected transcription repressor activity. When applied to zebrafish embryos, PICCORO permitted regulation of the activity of the mutant No Tail in response to 472-nm light provided by a light-emitting diode.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping