PUBLICATION

Functional analysis of limb enhancers in the developing fin

Authors
Booker, B.M., Murphy, K.K., and Ahituv, N.
ID
ZDB-PUB-131024-21
Date
2013
Source
Development genes and evolution   223(6): 395-399 (Journal)
Registered Authors
Ahituv, Nadav, Murphy, Karl
Keywords
enhancers, fin, limb, mesenchyme, ZPA, AER
MeSH Terms
  • Animal Fins/embryology*
  • Animals
  • Biological Evolution*
  • Gene Expression Regulation, Developmental*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
24068387 Full text @ Dev. Genes Evol.
Abstract

Despite diverging <365 million years ago, tetrapod limbs and pectoral fins express similar genes that could be regulated by shared regulatory elements. In this study, we set out to analyze the ability of enhancers to maintain tissue specificity in these two divergent structures. We tested 22 human sequences that were previously reported as mouse limb enhancers for their enhancer activity in zebrafish (Danio rerio). Using a zebrafish enhancer assay, we found that 10/22 (45 %) were positive for pectoral fin activity. Analysis of the various criteria that correlated with positive fin activity found that both spatial limb activity and evolutionary conservation are not good predictors of fin enhancer activity. These results suggest that zebrafish enhancer assays may be limited in detecting human limb enhancers, and this limitation does not improve by the use of limb spatial expression or evolutionary conservation.

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes