PUBLICATION

rbm 47, a novel RNA binding protein, regulates zebrafish head development

Authors
Guan, R., Ei-Rass, S., Spillane, D., Lam, S., Wang, Y., Wu, J., Chen, Z., Wang, A., Jia, Z., Keating, A., Hu, J., and Wen, X.Y.
ID
ZDB-PUB-130927-7
Date
2013
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   242(12): 1395-404 (Journal)
Registered Authors
Keywords
RNA binding protein, rbm47, head development, zebrafish, gene regulation, gene knockdown, morpholino, microarray
MeSH Terms
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Animals
  • Base Sequence
  • Head/embryology*
  • Molecular Sequence Data
  • Humans
  • Morpholinos/genetics
  • Wnt Proteins/metabolism
  • Wnt Signaling Pathway/genetics
  • Wnt Signaling Pathway/physiology*
  • Cytoskeletal Proteins/metabolism
  • Amino Acid Sequence
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Real-Time Polymerase Chain Reaction
  • Microarray Analysis
  • Gene Knockdown Techniques
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • DNA Primers/genetics
  • RNA-Binding Proteins/genetics
  • RNA-Binding Proteins/metabolism*
  • Zebrafish/embryology*
  • In Situ Hybridization
(all 25)
PubMed
24038582 Full text @ Dev. Dyn.
Abstract

Background: Vertebrate trunk induction requires inhibition of bone morphogenetic protein (BMP) signaling, whereas vertebrate head induction requires concerted inhibition of both Wnt and BMP signaling. RNA binding proteins play diverse roles in embryonic development and their roles in vertebrate head development remain to be elucidated.

Results: We first characterized the human RBM47 as a RNA binding protein that specifically binds RNA but not single stranded DNA. Next, we knocked down rbm47 gene function in zebrafish using morpholinos targeting the start codon and exon-1/intron-1 splice junction. Down-regulation of rbm47 resulted in headless and small head phenotypes, which can be rescued by a wnt8a blocking morpholino. To further reveal the mechanism of rbm47's role in head development, microarrays were performed to screen genes differentially expressed in normal and knockdown embryos. epcam and a2ml were identified as the most significantly up- and down-regulated genes, respectively. The microarrays also confirmed up-regulation of a number of genes involved in head development, including gsk3a, otx2 and chordin, which are important regulators of Wnt signaling.

Conclusion: Altogether, our findings reveal that Rbm47 is a novel RNA-binding protein critical for head formation and embryonic patterning during zebrafish embryogenesis which may act through a Wnt8a signaling pathway.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
Target Reagent Reagent Type
rbm47MO1-rbm47MRPHLNO
rbm47MO2-rbm47MRPHLNO
wnt8aMO1-wnt8aMRPHLNO
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Fish
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Antibodies
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Orthology
Gene Orthology
rbm47
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Engineered Foreign Genes
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Mapping
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