rbm 47, a novel RNA binding protein, regulates zebrafish head development
- Authors
- Guan, R., Ei-Rass, S., Spillane, D., Lam, S., Wang, Y., Wu, J., Chen, Z., Wang, A., Jia, Z., Keating, A., Hu, J., and Wen, X.Y.
- ID
- ZDB-PUB-130927-7
- Date
- 2013
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 242(12): 1395-404 (Journal)
- Registered Authors
- Keywords
- RNA binding protein, rbm47, head development, zebrafish, gene regulation, gene knockdown, morpholino, microarray
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cytoskeletal Proteins/metabolism
- DNA Primers/genetics
- Gene Expression Regulation, Developmental/genetics
- Gene Expression Regulation, Developmental/physiology*
- Gene Knockdown Techniques
- Head/embryology*
- Humans
- In Situ Hybridization
- Microarray Analysis
- Molecular Sequence Data
- Morpholinos/genetics
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism*
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Wnt Proteins/metabolism
- Wnt Signaling Pathway/genetics
- Wnt Signaling Pathway/physiology*
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 24038582 Full text @ Dev. Dyn.
Background: Vertebrate trunk induction requires inhibition of bone morphogenetic protein (BMP) signaling, whereas vertebrate head induction requires concerted inhibition of both Wnt and BMP signaling. RNA binding proteins play diverse roles in embryonic development and their roles in vertebrate head development remain to be elucidated.
Results: We first characterized the human RBM47 as a RNA binding protein that specifically binds RNA but not single stranded DNA. Next, we knocked down rbm47 gene function in zebrafish using morpholinos targeting the start codon and exon-1/intron-1 splice junction. Down-regulation of rbm47 resulted in headless and small head phenotypes, which can be rescued by a wnt8a blocking morpholino. To further reveal the mechanism of rbm47's role in head development, microarrays were performed to screen genes differentially expressed in normal and knockdown embryos. epcam and a2ml were identified as the most significantly up- and down-regulated genes, respectively. The microarrays also confirmed up-regulation of a number of genes involved in head development, including gsk3a, otx2 and chordin, which are important regulators of Wnt signaling.
Conclusion: Altogether, our findings reveal that Rbm47 is a novel RNA-binding protein critical for head formation and embryonic patterning during zebrafish embryogenesis which may act through a Wnt8a signaling pathway.