PUBLICATION

Normal Function of Myf5 During Gastrulation Is Required for Pharyngeal Arch Cartilage Development in Zebrafish Embryos

Authors
Lin, C.Y., Lee, H.C., Chen, H.C., Hsieh, C.C., and Tsai, H.J.
ID
ZDB-PUB-130905-15
Date
2013
Source
Zebrafish   10(4): 486-99 (Journal)
Registered Authors
Tsai, Huai-Jen
Keywords
none
MeSH Terms
  • Animals
  • Branchial Region/embryology*
  • Cartilage, Articular/growth & development*
  • Chondrogenesis
  • Embryonic Development*
  • Gene Knockdown Techniques
  • Head/embryology
  • Muscles/embryology
  • Myogenic Regulatory Factor 5/deficiency
  • Myogenic Regulatory Factor 5/physiology*
  • Neural Crest/embryology
  • Signal Transduction/physiology
  • Zebrafish/embryology*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/physiology*
PubMed
23992145 Full text @ Zebrafish
Abstract

Myf5, a myogenic regulatory factor, plays a key role in regulating muscle differentiation. However, it is not known if Myf5 has a regulatory role during early embryogenesis. Here, we used myf5-morpholino oligonucleotides [MO] to knock down myf5 expression and demonstrated a series of results pointing to the functional roles of Myf5 during early embryogenesis: (1) reduced head size resulting from abnormal morphology in the cranial skeleton; (2) decreased expressions of the cranial neural crest (CNC) markers foxd3, sox9a, dlx2, and col2a1; (3) defect in the chondrogenic neural crest similar to that of fgf3 morphants; (4) reduced fgf3/fgf8 transcripts in the cephalic mesoderm rescued by co-injection of myf5 wobble-mismatched mRNA together with myf5-MO1 during 12 h postfertilization; (5) abnormal patterns of axial and non-axial mesoderm causing expansion of the dorsal organizer, and (6) increased bmp4 gradient, but reduced fgf3/fgf8 marginal gradient, during gastrulation. Interestingly, overexpression of fgf3 could rescue the cranial cartilage defects caused by myf5-MO1, suggesting that Myf5 modulates craniofacial cartilage development through the fgf3 signaling pathway. Together, the loss of Myf5 function results in a cascade effect that begins with abnormal formation of the dorsal organizer during gastrulation, causing, in turn, defects in the CNC and cranial cartilage of myf5-knockdown embryos.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes