ZFIN ID: ZDB-PUB-130805-11
Quantitative Proteomics Reveals Diverse Roles of miR-148a from Gastric Cancer Progression to Neurological Development
Hu, C.W., Tseng, C.W., Chien, C.W., Huang, H.C., Ku, W.C., Lee, S.J., Chen, Y.J., and Juan, H.F.
Date: 2013
Source: Journal of Proteome Research   12(9): 3993-4004 (Journal)
Registered Authors: Lee, Shyh-Jye
Keywords: miR-148a, iTRAQ, proteome, neurological development, zebrafish
MeSH Terms:
  • Alzheimer Disease/genetics
  • Alzheimer Disease/metabolism
  • Animals
  • Base Sequence
  • Brain/embryology
  • Brain/metabolism
  • Case-Control Studies
  • Cell Line, Tumor
  • Disease Progression
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs/physiology*
  • Parkinson Disease/genetics
  • Parkinson Disease/metabolism
  • Proteomics
  • RNA Interference
  • Sequence Homology, Nucleic Acid
  • Spinal Cord/embryology
  • Spinal Cord/metabolism
  • Stomach Neoplasms
  • Transcriptome*
  • Zebrafish
PubMed: 23869555 Full text @ J. Proteome Res.

MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins were considered to be regulated by miR-148a. We found that not only proteins associated with cancer progression but also molecules involved in neural development were regulated by miR-148a. This study is the first to identify the function of miR-148a in neural development by using a proteomic approach. Analysis of a public clinical database also showed that the patients with neural diseases could display abnormal expression of miR-148a. Moreover, silencing of miR-148a led to the abnormal morphology and decreased expression of neuron-related markers in the developing brain of zebrafish. These results provided important insight into the regulation of neurological development elicited by miR-148a.