PUBLICATION

A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

Authors
Lim, S., Wang, Y., Yu, X., Huang, Y., Featherstone, M.S., and Sampath, K.
ID
ZDB-PUB-130710-114
Date
2013
Source
Genome biology   14(7): R69 (Journal)
Registered Authors
Featherstone, Mark, Lim, Shi Min, Sampath, Karuna, Wang, Yin
Keywords
none
MeSH Terms
  • Alleles
  • Animals
  • Base Sequence
  • Chromosome Deletion*
  • Crosses, Genetic
  • Deoxyribonucleases/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Female
  • Genetic Loci
  • Genomics/methods*
  • Germ-Line Mutation/genetics
  • Green Fluorescent Proteins/metabolism
  • Inheritance Patterns/genetics*
  • Male
  • Molecular Sequence Data
  • RNA/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zinc Fingers/genetics
PubMed
23815890 Full text @ Genome Biol.
Abstract

Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping