ZFIN ID: ZDB-PUB-130710-114
A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases
Lim, S., Wang, Y., Yu, X., Huang, Y., Featherstone, M.S., and Sampath, K.
Date: 2013
Source: Genome biology   14(7): R69 (Journal)
Registered Authors: Featherstone, Mark, Lim, Shi Min, Sampath, Karuna, Wang, Yin
Keywords: none
MeSH Terms:
  • Alleles
  • Animals
  • Base Sequence
  • Chromosome Deletion*
  • Crosses, Genetic
  • Deoxyribonucleases/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Female
  • Genetic Loci
  • Genomics/methods*
  • Germ-Line Mutation/genetics
  • Green Fluorescent Proteins/metabolism
  • Inheritance Patterns/genetics*
  • Male
  • Molecular Sequence Data
  • RNA/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zinc Fingers/genetics
PubMed: 23815890 Full text @ Genome Biol.

Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.