PUBLICATION

Enhancer and gene traps for molecular imaging and genetic analysis in zebrafish

Authors
Trinh, L.A., and Fraser, S.E.
ID
ZDB-PUB-130416-29
Date
2013
Source
Development, growth & differentiation   55(4): 434-45 (Review)
Registered Authors
Fraser, Scott E., Trinh, Le
Keywords
enhancer trap, gene trap, insertional mutagnesis, molecular imaging, protein trap, transposon
MeSH Terms
  • Animals
  • DNA Transposable Elements/genetics*
  • Enhancer Elements, Genetic*
  • Genetic Techniques
  • Genetic Vectors
  • Green Fluorescent Proteins/metabolism
  • Molecular Imaging/methods*
  • Mutagenesis*
  • Transcription Factors/metabolism
  • Transcriptome
  • Zebrafish
PubMed
23565993 Full text @ Dev. Growth Diff.
Abstract

Enhancer and gene trapping methods are highly effective means for the identification and functional analysis of transcriptionally active genes. With recent advances in fluorescent proteins and transposon based integration technologies, a growing family of trapping approaches has been developed in zebrafish, offering powerful tools to both visualize and functionally dissect gene networks during development. Coupled with the intrinsic advantages of the zebrafish model system, creative genetic engineering of trap vectors has enabled high-resolution molecular imaging and genetic manipulations. This review highlights the different enhancer and gene trap approaches that have been developed in zebrafish and offers insights into the strengths, limitations and experimental strategies for their application to enrich our knowledge of gene function and the cellular processes they control.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes