PUBLICATION

Zebrafish cilia

Authors
Drummond, I., and Austin-Tse, C.
ID
ZDB-PUB-130408-19
Date
2013
Source
Methods in enzymology   525: 219-244 (Chapter)
Registered Authors
Drummond, Iain
Keywords
zebrafish, cilia, morpholino, immunofluorescence, electron microscopy
MeSH Terms
  • Animals
  • Cilia/metabolism*
  • Cilia/physiology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Zebrafish
  • Zebrafish Proteins/metabolism*
PubMed
23522472 Full text @ Methods Enzymol.
Abstract

Zebrafish are ideally suited for analysis of genes required for ciliogenesis and cilia function. Combining genetic manipulation with high quality in vivo imaging, zebrafish embryos provide a high-throughput system for annotation of the cilia proteome. The specific advantages of the system are the availability of cilia mutants, the ability to target genes of unknown function using antisense methods, the feasibility of observing cilia in living embryos, and the ability to image fixed cilia in wholemount at high resolution. Techniques are described for analysis of mutants, gene knockdown using antisense morpholino oligos, visualizing cilia and cilia orientation in wholemount zebrafish embryos, live imaging cilia, and electron microscopy of zebrafish cilia.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping