PUBLICATION

miR-34 is maternally inherited in Drosophila melanogaster and Danio rerio

Authors
Soni, K., Choudhary, A., Patowary, A., Singh, A.R., Bhatia, S., Sivasubbu, S., Chandrasekaran, S., and Pillai, B.
ID
ZDB-PUB-130402-12
Date
2013
Source
Nucleic acids research   41(8): 4470-80 (Journal)
Registered Authors
Angom, Ramcharan Singh, Sivasubbu, Sridhar
Keywords
none
Datasets
GEO:GSE32360
MeSH Terms
  • Animals
  • Brain/embryology
  • Computational Biology
  • Drosophila Proteins/metabolism*
  • Drosophila Proteins/physiology
  • Drosophila melanogaster/embryology
  • Drosophila melanogaster/genetics*
  • Drosophila melanogaster/metabolism
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Inheritance Patterns
  • MicroRNAs/analysis
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • MicroRNAs/physiology*
  • Neurons/metabolism
  • Oocytes/chemistry
  • RNA Helicases/physiology
  • Ribonuclease III/physiology
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zygote/metabolism
PubMed
23470996 Full text @ Nucleic Acids Res.
Abstract

MicroRNAs (miRNAs) are small, endogenous, regulatory RNA molecules that can bind to partially complementary regions on target messenger RNAs and impede their expression or translation. We rationalized that miRNAs, being localized to the cytoplasm, will be maternally inherited during fertilization and may play a role in early development. Although Dicer is known to be essential for the transition from single-celled zygote to two-cell embryo, a direct role for miRNAs has not yet been demonstrated. We identified miRNAs with targets in zygotically expressed transcripts in Drosophila using a combination of transcriptome analysis and miRNA target prediction. We experimentally established that Drosophila miRNA dme-miR-34, the fly homologue of the cancer-related mammalian miRNA miR-34, involved in somatic-cell reprogramming and having critical role in early neuronal differentiation, is present in Drosophila embryos before initiation of zygotic transcription. We also show that the Drosophila miR-34 is dependent on maternal Dicer-1 for its expression in oocytes. Further, we show that miR-34 is also abundant in unfertilized oocytes of zebrafish. Its temporal expression profile during early development showed abundant expression in unfertilized oocytes that gradually decreased by 5 days post-fertilization (dpf). We find that knocking down the maternal, but not the zygotic, miR-34 led to developmental defects in the neuronal system during early embryonic development in zebrafish. Here, we report for the first time, the maternal inheritance of an miRNA involved in development of the neuronal system in a vertebrate model system.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping