Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs
- Authors
- Zhang, Y., Duc, A.C., Rao, S., Sun, X.L., Bilbee, A.N., Rhodes, M., Li, Q., Kappes, D.J., Rhodes, J., and Wiest, D.L.
- ID
- ZDB-PUB-130312-35
- Date
- 2013
- Source
- Developmental Cell 24(4): 411-425 (Journal)
- Registered Authors
- Li, Qin, Rhodes, Jennifer, Wiest, David
- Keywords
- none
- MeSH Terms
-
- Ribosomes/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Smad1 Protein/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Gene Expression Regulation, Developmental*
- Blotting, Western
- Cell Lineage*
- RNA, Messenger/genetics
- Thymus Gland/cytology
- Thymus Gland/metabolism
- Ribosomal Proteins/genetics
- Ribosomal Proteins/metabolism*
- Cell Differentiation
- Real-Time Polymerase Chain Reaction
- Hematopoietic Stem Cells/cytology*
- Hematopoietic Stem Cells/metabolism
- T-Lymphocytes/cytology*
- T-Lymphocytes/metabolism
- Core Binding Factor Alpha 2 Subunit/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Animals
- PubMed
- 23449473 Full text @ Dev. Cell
It remains controversial whether the highly homologous ribosomal protein (RP) paralogs found in lower eukaryotes have distinct functions and this has not been explored in vertebrates. Here we demonstrate that despite ubiquitous expression, the RP paralogs, Rpl22 and Rpl22-like1 (Rpl22l1) play essential, distinct, and antagonistic roles in hematopoietic development. Knockdown of Rpl22 in zebrafish embryos selectively blocks the development of T lineage progenitors after they have seeded the thymus. In contrast, knockdown of the Rpl22 paralog, Rpl22l1, impairs the emergence of hematopoietic stem cells (HSC) in the aorta-gonad-mesonephros by abrogating Smad1 expression and the consequent induction of essential transcriptional regulator, Runx1. Indeed, despite the ability of both paralogs to bind smad1 RNA, Rpl22 and Rpl22l1 have opposing effects on Smad1 expression. Accordingly, circumstances that tip the balance of these paralogs in favor of Rpl22 (e.g., Rpl22l1 knockdown or Rpl22 overexpression) result in repression of Smad1 and blockade of HSC emergence.