PUBLICATION
Mechanistic insights into the effect of nanoparticles on zebrafish hatch
- Authors
- Ong, K., Zhao, X., Thistle, M., Maccormack, T.J., Clark, R.J., Ma, G., Martinez-Rubi, Y., Simard, B., Loo, J., Veinot, J.G., and Goss, G.G.
- ID
- ZDB-PUB-130308-26
- Date
- 2014
- Source
- Nanotoxicology 8: 295-304 (Journal)
- Registered Authors
- Goss, Greg
- Keywords
- hatching enzyme, nanoparticle, hatch, ZHE1, protein interaction
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/chemistry
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/pathology
- Embryo, Nonmammalian/physiology
- Metal Nanoparticles/toxicity*
- Metals, Heavy/toxicity*
- Nanotubes, Carbon/toxicity
- Peptide Hydrolases/metabolism
- Silicon/toxicity
- Zebrafish/physiology*
- PubMed
- 23421642 Full text @ Nanotoxicology
Citation
Ong, K., Zhao, X., Thistle, M., Maccormack, T.J., Clark, R.J., Ma, G., Martinez-Rubi, Y., Simard, B., Loo, J., Veinot, J.G., and Goss, G.G. (2014) Mechanistic insights into the effect of nanoparticles on zebrafish hatch. Nanotoxicology. 8:295-304.
Abstract
Aquatic organisms are susceptible to waterborne nanoparticles (NP) and there is only limited understanding of the mechanisms by which these emerging contaminants may affect biological processes. This study used silicon (nSi), cadmium selenide (nCdSe), silver (nAg) and zinc NPs (nZnO) as well as single-walled carbon nanotubes (SWCNT) to assess NP effects on zebrafish (Danio rerio) hatch. Exposure of 10 mg/L nAg and nCdSe delayed zebrafish hatch and 100 mg/L of nCdSe as well as 10 and 100 mg/L of uncoated nZnO completely inhibited hatch and the embryos died within the chorion. Both the morphology and the movement of the embryos were not affected, and it was determined that the main mechanism of hatch inhibition by NPs is likely through the interaction of NPs with the zebrafish hatching enzyme. Furthermore, it was concluded that the observed effects arose from the NPs themselves and not their dissolved metal components.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping