PUBLICATION
Patch-clamp recordings from lateral line neuromast hair cells of the living zebrafish
- Authors
- Ricci, A.J., Bai, J.P., Song, L., Lv, C., Zenisek, D., and Santos-Sacchi, J.
- ID
- ZDB-PUB-130307-3
- Date
- 2013
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 33(7): 3131-3134 (Journal)
- Registered Authors
- Lv, Caixia, Zenisek, David
- Keywords
- none
- MeSH Terms
-
- Animals
- Female
- Functional Laterality/physiology
- Gap Junctions/physiology
- Hair Cells, Auditory/physiology*
- Larva
- Lateral Line System/innervation*
- Lateral Line System/physiology*
- Male
- Neurons/physiology*
- Patch-Clamp Techniques*
- Potassium Channels/physiology
- Synaptic Vesicles/physiology
- Zebrafish
- PubMed
- 23407967 Full text @ J. Neurosci.
Citation
Ricci, A.J., Bai, J.P., Song, L., Lv, C., Zenisek, D., and Santos-Sacchi, J. (2013) Patch-clamp recordings from lateral line neuromast hair cells of the living zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(7):3131-3134.
Abstract
Zebrafish are popular models for biological discovery. For investigators of the auditory and vestibular periphery, manipulations of hair cell and synaptic mechanisms have relied on inferences from extracellular recordings of physiological activity. We now provide data showing that hair cells and supporting cells of the lateral line can be directly patch-clamped, providing the first recordings of ionic channel activity, synaptic vesicle release, and gap junctional coupling in the neuromasts of living fish. Such capabilities will allow more detailed understanding of mechano-sensation of the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping