PUBLICATION
Generating parabiotic zebrafish embryos for cell migration and homing studies
- Authors
- Demy, D.L., Ranta, Z., Giorgi, J.M., Gonzalez, M., Herbomel, P., and Kissa, K.
- ID
- ZDB-PUB-130218-5
- Date
- 2013
- Source
- Nature Methods 10(3): 256-258 (Journal)
- Registered Authors
- Demy, Doris-Lou, Giorgi, Jean-Michel, Gonzalez, Magali, Herbomel, Philippe, Kissa-Marin, Karima
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement/physiology*
- Cell Tracking/methods*
- Embryo, Nonmammalian*
- Hematopoietic Stem Cells/physiology*
- Microscopy, Confocal
- Microscopy, Fluorescence
- Parabiosis/methods*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology
- PubMed
- 23377378 Full text @ Nat. Methods
Citation
Demy, D.L., Ranta, Z., Giorgi, J.M., Gonzalez, M., Herbomel, P., and Kissa, K. (2013) Generating parabiotic zebrafish embryos for cell migration and homing studies. Nature Methods. 10(3):256-258.
Abstract
Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping