PUBLICATION

The transcription factor Vox represses endoderm development by interacting with Casanova and Pou2

Authors
Zhao, J., Lambert, G., Meijer, A.H., and Rosa, F.M.
ID
ZDB-PUB-130211-10
Date
2013
Source
Development (Cambridge, England)   140(5): 1090-1099 (Journal)
Registered Authors
Lambert, Guillaume, Meijer, Annemarie H., Rosa, Frederic, Zhao, Jue
Keywords
none
MeSH Terms
  • Animals, Genetically Modified
  • Nodal Signaling Ligands/genetics
  • Nodal Signaling Ligands/metabolism
  • Nodal Signaling Ligands/physiology
  • Protein Interaction Domains and Motifs/genetics
  • Protein Interaction Domains and Motifs/physiology
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • SOX Transcription Factors/metabolism*
  • SOX Transcription Factors/physiology
  • Octamer Transcription Factor-3/metabolism*
  • Octamer Transcription Factor-3/physiology
  • Homeodomain Proteins/chemistry
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Homeodomain Proteins/physiology*
  • Protein Binding/physiology
  • Sequence Deletion
  • Down-Regulation/genetics
  • Animals
  • Endoderm/embryology*
  • Endoderm/growth & development
  • Endoderm/metabolism
  • Tissue Distribution
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Bone Morphogenetic Proteins/physiology
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zebrafish Proteins/physiology*
  • Embryo, Nonmammalian
  • Repressor Proteins/chemistry
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism*
  • Repressor Proteins/physiology*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription Factors/physiology
(all 41)
PubMed
23364327 Full text @ Development
Abstract

Endoderm and mesoderm are both formed upon activation of Nodal signaling but how endoderm differentiates from mesoderm is still poorly explored. The sox-related gene casanova (sox32) acts downstream of the Nodal signal, is essential for endoderm development and requires the co-factor Pou2 (Pou5f1, Oct3, Oct4) in this process. Conversely, BMP signals have been shown to inhibit endoderm development by an as yet unexplained mechanism. In a search for Casanova regulators in zebrafish, we identified two of its binding partners as the transcription factors Pou2 and Vox, a member of the Vent group of proteins also involved in the patterning of the gastrula. In overexpression studies we show that vox and/or Vent group genes inhibit the capacity of Casanova to induce endoderm, even in the presence of its co-factor Pou2, and that Vox acts as a repressor in this process. We further show that vox, but not other members of the Vent group, is essential for defining the proper endodermal domain size at gastrulation. In this process, vox acts downstream of BMPs. Cell fate analysis further shows that Vox plays a key role downstream of BMP signals in regulating the capacity of Nodal to induce endoderm versus mesoderm by modulating the activity of the Casanova/Pou2 regulatory system.

Genes / Markers
Figures
Figure Gallery (7 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
vedMO1-vedMRPHLNO
ventMO1-ventMRPHLNO
voxMO1-voxMRPHLNO
1 - 3 of 3
Show
Fish
1 - 2 of 2
Show
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-mycmonoclonal
    IgG1Mouse
    1 - 1 of 1
    Show
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping