ZFIN ID: ZDB-PUB-130207-7
Plasma membrane Ca(2+) ATPase Atp2b1a regulates bone mineralization in zebrafish
Go, W., and Korzh, V.
Date: 2013
Source: Bone   54(1): 48-57 (Journal)
Registered Authors: Go, William, Korzh, Vladimir
Keywords: plasma membrane Ca2 + pump, pharyngeal epithelium, calcification, ameloblasts, dlx2b
MeSH Terms:
  • Ameloblasts/metabolism
  • Animals
  • Bone and Bones/embryology
  • Bone and Bones/metabolism
  • Calcification, Physiologic*
  • Epithelium/embryology
  • Epithelium/enzymology
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Green Fluorescent Proteins/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Odontogenesis
  • Osteogenesis
  • Pharynx/embryology
  • Pharynx/enzymology
  • Plasma Membrane Calcium-Transporting ATPases/genetics
  • Plasma Membrane Calcium-Transporting ATPases/metabolism*
  • Tooth Calcification
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription, Genetic
  • Transgenes/genetics
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 23353107 Full text @ Bone

The zebrafish transgenic lines provide a possibility to observe the development of tissues and organs in real time. Using the reporter line for the zebrafish plasma membrane Ca2 + ATPase (SqET4), we detected its expression in the epithelium of pharyngeal teeth and analyzed its role in their calcification and that of cranial bones. atp2b1a's expression in the pharyngeal epithelium is faithfully recapitulated in the SqET4 transgenics by GFP expression. We showed by morpholino knockdown of Atp2b1a translations as well as chemical inhibition of Atp2b1a pump activity using carboxyeosin, that its activity is required to facilitate calcification of the developing pharyngeal teeth by the dental epithelium. Atp2b1a could be required during calcification of endochondral bones, where it acts at two levels: 1) by exporting Ca2 + from ameloblasts, it provides raw material for calcifying the pharyngeal teeth by adjacent odontoblasts; and 2) by regulating terminal differentiation of pharyngeal epithelial cells, including ameloblasts required for tissue hyper-mineralization. atp2b1a's expression in the pharyngeal epithelium is regulated by the homeodomain transcription factor dlx2b.