PUBLICATION

Identification of TNF13b (BAFF) gene from grass carp (Ctenopharyngodon idella) and its immune response to bacteria and virus

Authors
Pandit, N.P., Shen, Y., Wang, W., Chen, Y., and Li, J.
ID
ZDB-PUB-130207-5
Date
2013
Source
Developmental and comparative immunology   39(4): 460-464 (Journal)
Registered Authors
Keywords
grass carp, BAFF, Aeromonas hydrophilia, Aquareovirus, cDNA cloning, mRNA expression
MeSH Terms
  • Aeromonas hydrophila/immunology
  • Amino Acid Sequence
  • Animals
  • B-Cell Activating Factor/chemistry
  • B-Cell Activating Factor/genetics*
  • B-Cell Activating Factor/immunology*
  • Base Sequence
  • Carps/genetics*
  • Carps/immunology*
  • Carps/microbiology
  • Carps/virology
  • Cloning, Molecular
  • DNA, Complementary
  • Fish Diseases/immunology*
  • Fish Diseases/microbiology
  • Fish Diseases/virology
  • Fish Proteins/chemistry
  • Fish Proteins/genetics*
  • Fish Proteins/immunology*
  • Gene Expression Profiling
  • Gram-Negative Bacterial Infections/immunology
  • Gram-Negative Bacterial Infections/veterinary
  • Phylogeny
  • Reoviridae/immunology
  • Reoviridae Infections/immunology
  • Reoviridae Infections/veterinary
  • Sequence Alignment
  • Sequence Analysis, DNA
PubMed
23352623 Full text @ Dev. Comp. Immunol.
Abstract

The TNF superfamily B cell activating factor (BAFF) is a central cytokine in several diseases. A BAFF gene has been cloned from grass carp (Ctenopharyngodon idella), analyzed its structure, and investigated its expression pattern in various tissues after Aeromonas hydrophila and Aquareovirus infection. The open reading frame of grass carp BAFF (gcBAFF) consists of 804 bases encoding 267 amino acids. Phylogenetic analysis shows the gcBAFF is most closely related to other teleost BAFFs with the highest similarity to zebrafish. RT-PCR analysis shows the gcBAFF transcript is expressed in a wide range of tissues with the highest expression in skin and spleen. Upon induction by A. hydrophila and Aquareovirus, its expression is significantly up-regulated in gill, liver, kidney, spleen and skin as compared to PBS injected fish. The association of increased BAFF expression after bacterial and viral infections suggests that it plays a potentially important role in immune system of fish.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping