ZFIN ID: ZDB-PUB-121227-13
Proteomic and functional analysis of zebrafish after administration of antimicrobial peptide epinecidin-1
Huang, T.C., and Chen, J.Y.
Date: 2013
Source: Fish & shellfish immunology   34(2): 593-598 (Journal)
Registered Authors: Chen, Jyh-Yih
Keywords: proteomic, zebrafish, epinecidin-1, antimicrobial peptide
MeSH Terms:
  • Animals
  • Antimicrobial Cationic Peptides/administration & dosage
  • Antimicrobial Cationic Peptides/pharmacology*
  • Cytoskeleton/drug effects
  • Cytoskeleton/physiology*
  • DNA Primers/genetics
  • Electrophoresis, Gel, Two-Dimensional/veterinary
  • Fish Proteins/administration & dosage
  • Fish Proteins/pharmacology*
  • Gene Expression Regulation/drug effects*
  • Immunity, Innate/immunology*
  • Male
  • Mass Spectrometry/veterinary
  • Proteomics/methods
  • Real-Time Polymerase Chain Reaction/veterinary
  • Reverse Transcriptase Polymerase Chain Reaction/veterinary
  • Zebrafish/immunology*
  • Zebrafish/metabolism
PubMed: 23261508 Full text @ Fish Shellfish Immunol.

Antimicrobial peptides (AMPs) play important roles in innate immunity. One such AMP, epinecidin-1, exhibits antibacterial effects in zebrafish. In the current study, we aimed to identify the antimicrobial-associated proteins affected by epinecidin-1 treatment, and to unravel the underlying antimicrobial molecular mechanisms of epinecidin-1. We analyzed proteome changes in epinecidin-1-treated zebrafish using two-dimensional electrophoresis (2DE) coupled to mass spectrometry. Several differentially expressed proteins were identified, some of which were validated by real-time quantitative RT-PCR. The differentially expressed proteins were mapped onto Ingenuity Pathway Analysis canonical pathways, to construct a possible protein–protein interacting network regulated by epinecidin-1; this network suggested a potential role of epinecindin-1 in cytoskeletal assembly and organization. Our findings imply that epinecidin-1 may stabilize the cytoskeleton network in host cells, thereby promoting resistance to bacterial infection.