PUBLICATION

Proteomic and functional analysis of zebrafish after administration of antimicrobial peptide epinecidin-1

Authors
Huang, T.C., and Chen, J.Y.
ID
ZDB-PUB-121227-13
Date
2013
Source
Fish & shellfish immunology   34(2): 593-598 (Journal)
Registered Authors
Chen, Jyh-Yih
Keywords
proteomic, zebrafish, epinecidin-1, antimicrobial peptide
MeSH Terms
  • Electrophoresis, Gel, Two-Dimensional/veterinary
  • Proteomics/methods
  • Zebrafish/immunology*
  • Zebrafish/metabolism
  • Male
  • DNA Primers/genetics
  • Reverse Transcriptase Polymerase Chain Reaction/veterinary
  • Fish Proteins/administration & dosage
  • Fish Proteins/pharmacology*
  • Immunity, Innate/immunology*
  • Animals
  • Antimicrobial Cationic Peptides/administration & dosage
  • Antimicrobial Cationic Peptides/pharmacology*
  • Gene Expression Regulation/drug effects*
  • Real-Time Polymerase Chain Reaction/veterinary
  • Cytoskeleton/drug effects
  • Cytoskeleton/physiology*
  • Mass Spectrometry/veterinary
(all 18)
PubMed
23261508 Full text @ Fish Shellfish Immunol.
Abstract

Antimicrobial peptides (AMPs) play important roles in innate immunity. One such AMP, epinecidin-1, exhibits antibacterial effects in zebrafish. In the current study, we aimed to identify the antimicrobial-associated proteins affected by epinecidin-1 treatment, and to unravel the underlying antimicrobial molecular mechanisms of epinecidin-1. We analyzed proteome changes in epinecidin-1-treated zebrafish using two-dimensional electrophoresis (2DE) coupled to mass spectrometry. Several differentially expressed proteins were identified, some of which were validated by real-time quantitative RT-PCR. The differentially expressed proteins were mapped onto Ingenuity Pathway Analysis canonical pathways, to construct a possible protein–protein interacting network regulated by epinecidin-1; this network suggested a potential role of epinecindin-1 in cytoskeletal assembly and organization. Our findings imply that epinecidin-1 may stabilize the cytoskeleton network in host cells, thereby promoting resistance to bacterial infection.

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