PUBLICATION
Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues
- Authors
- Lagendijk, A.K., Moulton, J.D., and Bakkers, J.
- ID
- ZDB-PUB-121210-7
- Date
- 2012
- Source
- Biology Open 1(6): 566-569 (Journal)
- Registered Authors
- Bakkers, Jeroen, Moulton, Jon D.
- Keywords
- microRNA, in situ hybridization, zebrafish
- MeSH Terms
- none
- PubMed
- 23213449 Full text @ Biol. Open
Citation
Lagendijk, A.K., Moulton, J.D., and Bakkers, J. (2012) Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biology Open. 1(6):566-569.
Abstract
Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression is essential. Here, we provide a detailed protocol for miRNA whole mount in situ hybridization. We introduce the use of Morpholino based oligos as antisense probes for miRNA detection, in addition to the current “gold standard” locked nucleic acid (LNA) probes. Furthermore we have modified existing miRNA in situ protocols thereby improving both sensitivity and resolution of miRNA visualization in whole zebrafish embryos and adult tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping