PUBLICATION

Functional expression of SCL/TAL1 interrupting locus (Stil) protects retinal dopaminergic cells from neurotoxin-induced degeneration

Authors
Li, J., Li, P., Carr, A., Wang, X., Delapaz, A., Sun, L., Lee, E., Tomei, E., and Li, L.
ID
ZDB-PUB-121205-32
Date
2013
Source
The Journal of biological chemistry   288(2): 886-893 (Journal)
Registered Authors
Li, Lei
Keywords
cell death, neurons, neurotoxin, retina, zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Primers
  • Dopamine/metabolism*
  • Hedgehog Proteins/metabolism
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Mutation
  • Neurons/cytology
  • Neurons/drug effects*
  • Neurons/metabolism
  • Neurotoxins/toxicity*
  • Oxidopamine/toxicity*
  • Retina/cytology
  • Retina/drug effects*
  • Retina/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Zebrafish/embryology
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology
PubMed
23166330 Full text @ J. Biol. Chem.
Abstract

We previously isolated a dominant mutation, night blindness b (nbb), which causes a late-onset of retinal dopaminergic cell degeneration in zebrafish. In this study, we cloned the zebrafish nbb locus. Sequencing results revealed that nbb is a homolog of the vertebrate SCL/TAL1 interrupting locus (Stil). The Stil gene has been shown to play important roles in the regulation of vertebrate embryonic neural development and human cancer cell proliferation. In this study, we demonstrate that functional expression of Stil is also required for neural survival in adult animals. In zebrafish, deficiency in the expression of Stil resulted in increased toxic susceptibility of retinal dopaminergic cells to the neurotoxin 6-hydroxydopamine. Increases in Stil-mediated Shh signaling transduction (i.e., by knocking down the Shh repressor Sufu) prevented dopaminergic cell death induced by neurotoxic insult. The data suggests that the oncogene Stil also plays important roles in neural protection.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping