Antiangiogenic Effects and Mechanisms of trans-Ethyl p-Methoxycinnamate from Kaempferia galanga L
- Authors
- He, Z.H., Yue, G.G., Lau, C.B., Ge, W., and But, P.P.
- ID
- ZDB-PUB-121120-4
- Date
- 2012
- Source
- Journal of Agricultural and Food Chemistry 60(45): 11309-11317 (Journal)
- Registered Authors
- Ge, Wei
- Keywords
- Kaempferia galanga, spice, antiangiogenesis, zebrafish, trans-ethyl p-methoxyciunnamate
- MeSH Terms
-
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Cell Proliferation/drug effects
- Cinnamates/chemistry
- Cinnamates/pharmacology*
- Human Umbilical Vein Endothelial Cells/cytology
- Human Umbilical Vein Endothelial Cells/drug effects
- Human Umbilical Vein Endothelial Cells/metabolism
- Humans
- Neovascularization, Physiologic/drug effects*
- Plant Extracts/chemistry
- Plant Extracts/pharmacology*
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish
- Zingiberaceae/chemistry*
- PubMed
- 23106130 Full text @ J. Agric. Food Chem.
Kaempferia galanga L. (Zingiberaceae) is an aromatic herb and a popular spice used as a condiment in Asian cuisine. The ethanol extract of the dried plant and its successive four subfractions were investigated on zebrafish model by quantitative endogenous alkaline phosphatase assay. Both n-hexane and ethyl acetate fractions had antiangiogenic activity, and two major active components (trans-ethyl p-methoxycinnamate and kaempferol) showed potent antiangiogenic effects on wild-type zebrafish. Because of its much stronger effect and no antiangiogenic activity reported, trans-ethyl p-methoxycinnamate was further investigated for its action mechanism. It dose dependently inhibited vessel formation on both wild- and Tg(fli1a:EGFP)y1-type zebrafish embryos. The semiquantitative reverse transcription polymerase chain reaction assay suggested that trans-ethyl p-methoxycinnamate affects multiple molecular targets related to angiogenesis. In vitro, it specifically inhibited the migration and tube formation of human umbilical vein endothelial cells. In vivo, it could block bFGF-induced vessel formation on Matrigel plug assay.