ZFIN ID: ZDB-PUB-121120-4
Antiangiogenic Effects and Mechanisms of trans-Ethyl p-Methoxycinnamate from Kaempferia galanga L
He, Z.H., Yue, G.G., Lau, C.B., Ge, W., and But, P.P.
Date: 2012
Source: Journal of Agricultural and Food Chemistry   60(45): 11309-11317 (Journal)
Registered Authors: Ge, Wei
Keywords: Kaempferia galanga, spice, antiangiogenesis, zebrafish, trans-ethyl p-methoxyciunnamate
MeSH Terms:
  • Angiogenesis Inhibitors/chemistry
  • Angiogenesis Inhibitors/pharmacology*
  • Animals
  • Cell Proliferation/drug effects
  • Cinnamates/chemistry
  • Cinnamates/pharmacology*
  • Human Umbilical Vein Endothelial Cells/cytology
  • Human Umbilical Vein Endothelial Cells/drug effects
  • Human Umbilical Vein Endothelial Cells/metabolism
  • Humans
  • Neovascularization, Physiologic/drug effects*
  • Plant Extracts/chemistry
  • Plant Extracts/pharmacology*
  • Vascular Endothelial Growth Factor A/metabolism
  • Zebrafish
  • Zingiberaceae/chemistry*
PubMed: 23106130 Full text @ J. Agric. Food Chem.
ABSTRACT

Kaempferia galanga L. (Zingiberaceae) is an aromatic herb and a popular spice used as a condiment in Asian cuisine. The ethanol extract of the dried plant and its successive four subfractions were investigated on zebrafish model by quantitative endogenous alkaline phosphatase assay. Both n-hexane and ethyl acetate fractions had antiangiogenic activity, and two major active components (trans-ethyl p-methoxycinnamate and kaempferol) showed potent antiangiogenic effects on wild-type zebrafish. Because of its much stronger effect and no antiangiogenic activity reported, trans-ethyl p-methoxycinnamate was further investigated for its action mechanism. It dose dependently inhibited vessel formation on both wild- and Tg(fli1a:EGFP)y1-type zebrafish embryos. The semiquantitative reverse transcription polymerase chain reaction assay suggested that trans-ethyl p-methoxycinnamate affects multiple molecular targets related to angiogenesis. In vitro, it specifically inhibited the migration and tube formation of human umbilical vein endothelial cells. In vivo, it could block bFGF-induced vessel formation on Matrigel plug assay.

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