PUBLICATION

Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system

Authors
Umeda, K., Shoji, W., Sakai, S., Muto, A., Kawakami, K., Ishizuka, T., and Yawo, H.
ID
ZDB-PUB-121016-19
Date
2013
Source
Neuroscience research   75(1): 69-75 (Journal)
Registered Authors
Kawakami, Koichi, Muto, Akira, Shoji, Wataru
Keywords
zebrafish, transgenic, Gal4-UAS, optogenetics, channelrhodopsin, Rohon-Beard neuron, escape behavior, swimming, somatosensory, photostimulation
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Behavior, Animal/physiology
  • Chimera
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism
  • HEK293 Cells
  • Humans
  • Immunohistochemistry
  • Mechanoreceptors/physiology*
  • Microscopy, Confocal
  • Optogenetics/methods*
  • Patch-Clamp Techniques
  • Rhodopsin/biosynthesis*
  • Rhodopsin/genetics
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
23044184 Full text @ Neurosci. Res.
Abstract

Channelrhodopsin (ChR)-wide receiver (ChRWR), one of the chimeric molecule of ChR1 and ChR2, has several advantages over ChR2 such as improved expression in the plasma membrane and enhanced photocurrent with small desensitization. Here we generated transgenic zebrafish (Danio rerio) expressing ChRWR as a conjugate of EGFP under the regulation of UAS promoter (UAS:ChRWR-EGFP). When crossed with a Gal4 line, SAGFF36B, ChRWR-EGFP was selectively expressed in primary mechanosensory Rohon–Beard (RB) neurons. The direct photoactivation of RB neurons was sufficient to trigger the escape behavior. The UAS:ChRWR-EGFP line could facilitate a variety of investigations of neural networks and behaviors of zebrafish in vivo.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes