PUBLICATION

Noise drives sharpening of gene expression boundaries in the zebrafish hindbrain

Authors
Zhang, L., Radtke, K., Zheng, L., Cai, A.Q., Schilling, T.F., and Nie, Q.
ID
ZDB-PUB-121005-20
Date
2012
Source
Molecular Systems Biology   8: 613 (Journal)
Registered Authors
Schilling, Tom, Zhang, Li-Kun
Keywords
cellular decision, intracellular noise, morphogen, signal transduction network, stochastic fluctuation
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental*/drug effects
  • Models, Biological
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology
  • Rhombencephalon/metabolism*
  • Signal Transduction*/drug effects
  • Signal Transduction*/genetics
  • Tretinoin/pharmacology
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
23010996 Full text @ Mol. Syst. Biol.
Abstract

Morphogens provide positional information for spatial patterns of gene expression during development. However, stochastic effects such as local fluctuations in morphogen concentration and noise in signal transduction make it difficult for cells to respond to their positions accurately enough to generate sharp boundaries between gene expression domains. During development of rhombomeres in the zebrafish hindbrain, the morphogen retinoic acid (RA) induces expression of hoxb1a in rhombomere 4 (r4) and krox20 in r3 and r5. Fluorescent in situ hybridization reveals rough edges around these gene expression domains, in which cells co-express hoxb1a and krox20 on either side of the boundary, and these sharpen within a few hours. Computational analysis of spatial stochastic models shows, surprisingly, that noise in hoxb1a/krox20 expression actually promotes sharpening of boundaries between adjacent segments. In particular, fluctuations in RA initially induce a rough boundary that requires noise in hoxb1a/krox20 expression to sharpen. This finding suggests a novel noise attenuation mechanism that relies on intracellular noise to induce switching and coordinate cellular decisions during developmental patterning.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping