Evaluating the Toxicity of Hydroxyapatite Nanoparticles in Catfish Cells and Zebrafish Embryos
- Authors
- Zhao, X., Ong, K.J., Ede, J.D., Stafford, J.L., Ng, K.W., Goss, G.G., and Loo, S.C.
- ID
- ZDB-PUB-120815-16
- Date
- 2013
- Source
- Small (Weinheim an der Bergstrasse, Germany) 9(9-10): 1734-41 (Journal)
- Registered Authors
- Goss, Greg
- Keywords
- nanoparticle shapes, hydroxypatite, nanotoxicology, zebrafish embryo, hatching
- MeSH Terms
-
- Animals
- B-Lymphocytes/drug effects*
- Catfishes*
- Dose-Response Relationship, Drug
- Durapatite/toxicity*
- Embryo, Nonmammalian/drug effects*
- In Vitro Techniques
- Microscopy, Electron, Transmission
- Nanoparticles/toxicity*
- T-Lymphocytes/drug effects*
- Zebrafish/embryology*
- PubMed
- 22887936 Full text @ Small
The toxicity of needle-(nHA-ND) and rod-shaped (nHA-RD) hydroxyapatite (HA) nanoparticles is evaluated in vitro on catfish B-cells (3B11) and catfish T-cells (28s.3) and in vivo on zebrafish embryos to determine if biological effects are similar to the effects seen in mammalian in vitro systems. Neither nHA-ND nor nHA-RD affect cell viability at concentrations of 10 to 300 μg mL1. However, 30 μg mL1 needle-shaped nHA lower metabolic activity of the cells. Axial deformations are seen in zebrafish exposed to 300 μg mL1 needle shaped nHA after 120 h. For the first time, nHA is reported to cause zebrafish hatching delay. The lowest concentration (3 μg mL1) of both types of nHA cause the highest hatching inhibition and needle-shaped nHA exposed zebrafish exhibit the lowest hatch at 72 h post fertilization.