ZFIN ID: ZDB-PUB-120807-40
Developmental Role of Zebrafish Protease-Activated Receptor 1 (PAR1) in the Cardio-Vascular System
Ellertsdottir, E., Berthold, P.R, Bouzaffour, M., Dufourcq, P., Trayer, V., Gauron, C., Vriz, S., Affolter, M., and Rampon, C.
Date: 2012
Source: PLoS One   7(7): e42131 (Journal)
Registered Authors: Affolter, Markus, Dufourcq, Pascale, Ellertsdottir, Elin, Vriz, Sophie
Keywords: Embryos, Zebrafish, Heart rate, Heart, Hemorrhage, Morpholino, Veins, Multiple alignment calculation
MeSH Terms:
  • Animals
  • Cardiovascular System/embryology*
  • Evolution, Molecular
  • Gene Knockdown Techniques
  • Heart Rate
  • Receptor, PAR-1/genetics
  • Receptor, PAR-1/physiology*
  • Regional Blood Flow
  • Zebrafish/embryology*
PubMed: 22860064 Full text @ PLoS One

Thrombin receptor, F2R or PAR1 is a G-protein coupled receptor, located in the membrane of endothelial cells. It has been initially found to transduce signals in hemostasis, but recently also known to act in cancer and in vascular development. Mouse embryos lacking PAR1 function die from hemorrhages with varying frequency at midgestation. We have performed a survey of potential PAR1 homologs in the zebrafish genome and identified a teleost ortholog of mammalian PAR1. Knockdown of par1 function in zebrafish embryos demonstrates a requirement for Par1 in cardio-vascular development. Furthermore, we show that function of Par1 requires the presence of a phylogenetically conserved proteolytic cleavage site and a second intracellular domain. Altogether our results demonstrate a high degree of conservation of PAR1 proteins in the vertebrate lineage in respect to amino acid sequence as well as protein function.