PUBLICATION

Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

Authors
Zeng, Y., Xu, J., Li, D., Li, L., Wen, Z., and Qu, J.Y.
ID
ZDB-PUB-120705-28
Date
2012
Source
Optics letters   37(13): 2490-2492 (Journal)
Registered Authors
Wen, Zilong
Keywords
none
MeSH Terms
  • Animals
  • Blood Circulation
  • Erythrocytes/cytology
  • Flow Cytometry/methods*
  • Leukocytes/cytology
  • Molecular Imaging/methods*
  • Photons*
  • Spectrometry, Fluorescence/methods*
  • Zebrafish*/physiology
PubMed
22743431 Full text @ Opt. Lett.
Abstract

We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes