PUBLICATION

Identification and Regulation of a Molecular Module for Bleb-Based Cell Motility

Authors
Goudarzi, M., Banisch, T.U., Mobin, M.B., Maghelli, N., Tarbashevich, K., Strate, I., van den Berg, J., Blaser, H., Bandemer, S., Paluch, E., Bakkers, J., Toli-Nørrelykke, I.M., and Raz, E.
ID
ZDB-PUB-120702-14
Date
2012
Source
Developmental Cell   23(1): 210-218 (Journal)
Registered Authors
Bakkers, Jeroen, Bandemer, Sabine, Blaser, Heiko, Goudarzi, Mehdi, Raz, Erez, Tarbashevich, Katsiyarina, van den Berg, Jana
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cell Adhesion/physiology
  • Cell Movement/physiology*
  • Cell Polarity/physiology
  • Germ Cells/cytology*
  • Germ Cells/physiology
  • Homeostasis/physiology
  • Hydrostatic Pressure
  • MicroRNAs/genetics
  • Molecular Sequence Data
  • Myosins/physiology
  • RNA-Binding Proteins/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed
22705393 Full text @ Dev. Cell
Abstract

Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restoring motility. We have defined myosin contractility, cell adhesion, and cortex properties as factors whose proper regulation is sufficient for restoring cell migration of this cell type. Tight control over the level of these cellular features, achieved through a balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping