ZFIN ID: ZDB-PUB-120531-10
Live imaging kidney development in zebrafish
Vasilyev, A., and Drummond, I.A.
Date: 2012
Source: Methods in molecular biology (Clifton, N.J.)   886: 55-70 (Chapter)
Registered Authors: Drummond, Iain, Vasilyev, Aleksandr
Keywords: zebrafish, live cell imaging, time lapse, kidney, migration
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/ultrastructure*
  • Female
  • Image Processing, Computer-Assisted/methods
  • Kidney/embryology*
  • Microscopy, Confocal/methods*
  • Morpholinos/metabolism
  • Zebrafish/embryology*
PubMed: 22639251 Full text @ Meth. Mol. Biol.
ABSTRACT

The zebrafish has emerged as a powerful model to study organ development and regeneration. It has a number of advantages over other vertebrate model systems. The embryo can be kept transparent throughout embryonic development, which allows direct visualization of the developing organs. In addition, embryos can be easily manipulated surgically, genetically, or chemically. Furthermore, because nephron shape and function are remarkably conserved among vertebrates, zebrafish findings can directly inform human studies. Here, we describe a simple procedure that can be used by laboratories to investigate the development of zebrafish kidney and other organs by time-lapse microscopy.

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