ZFIN ID: ZDB-PUB-120529-46
Long-Range Ca(2+) Waves Transmit Brain-Damage Signals to Microglia
Sieger, D., Moritz, C., Ziegenhals, T., Prykhozhij, S., and Peri, F.
Date: 2012
Source: Developmental Cell   22(6): 1138-1148 (Journal)
Registered Authors: Peri, Francesca, Prykhozhij, Sergey, Sieger, Dirk
Keywords: none
MeSH Terms:
  • Adenosine Triphosphate/physiology
  • Animals
  • Brain Injuries/physiopathology*
  • Calcium Signaling/drug effects
  • Calcium Signaling/physiology*
  • Cell Death/drug effects
  • Cell Death/physiology
  • Cell Movement/drug effects
  • Cell Movement/physiology
  • Chelating Agents/metabolism
  • Glutamic Acid/physiology
  • Laser Therapy
  • Microglia/drug effects
  • Microglia/physiology*
  • Zebrafish/growth & development
  • Zebrafish/physiology
PubMed: 22632801 Full text @ Dev. Cell

Microglia are the resident phagocytes of the brain that are responsible for the clearance of injured neurons, an essential step in subsequent tissue regeneration. How death signals are controlled both in space and time to attract these cells toward the site of injury is a topic of great interest. To this aim, we have used the optically transparent zebrafish larval brain and identified rapidly propagating Ca2+ waves that determine the range of microglial responses to neuronal cell death. We show that while Ca2+-mediated microglial responses require ATP, the spreading of intercellular Ca2+ waves is ATP independent. Finally, we identify glutamate as a potent inducer of Ca2+-transmitted microglial attraction. Thus, this real-time analysis reveals the existence of a mechanism controlling microglial targeted migration to neuronal injuries that is initiated by glutamate and proceeds across the brain in the form of a Ca2+ wave.