PUBLICATION
Functional and heterologous expression of human protein O-linked mannose beta-1,2-N-acetylglucosaminyltransferase 1 in zebrafish
- Authors
- Avsar-Ban, E., Ishikawa, H., Akiyama, S., Manya, H., Endo, T., and Tamaru, Y.
- ID
- ZDB-PUB-120529-4
- Date
- 2012
- Source
- Journal of bioscience and bioengineering 114(2): 237-239 (Journal)
- Registered Authors
- Tamaru, Yutaka
- Keywords
- zebrafish, membrane-associated protein, gene expression system, protein, O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 (POMGnT1), O-mannosylation, hatching enzyme 1 promoter
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Genetic Vectors/genetics
- Humans
- Membrane Proteins/biosynthesis
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- N-Acetylglucosaminyltransferases/biosynthesis
- N-Acetylglucosaminyltransferases/genetics*
- N-Acetylglucosaminyltransferases/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism*
- PubMed
- 22608994 Full text @ J. Biosci. Bioeng.
Citation
Avsar-Ban, E., Ishikawa, H., Akiyama, S., Manya, H., Endo, T., and Tamaru, Y. (2012) Functional and heterologous expression of human protein O-linked mannose beta-1,2-N-acetylglucosaminyltransferase 1 in zebrafish. Journal of bioscience and bioengineering. 114(2):237-239.
Abstract
Although membrane-associated proteins are related to many diseases and are important targets for drug discovery, their expression is often difficult in bacterial hosts such as Escherichia coli. To overcome this limitation, here, we focused on a novel host-vector system in zebrafish for the expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 (hPOMGnT1) which is related to muscle-eye-brain disease. For the expression of hPOMGnT1, the vector pZex-EGFP-pXI-hPOMGnT1 was constructed and injected into fertilized eggs. Using this system, we demonstrated that recombinant hPOMGnT1 was successfully expressed in the whole bodies of zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping