ZFIN ID: ZDB-PUB-120507-8
Oral administration of recombinant epinecidin-1 protected grouper (Epinephelus coioides) and zebrafish (Danio rerio) from Vibrio vulnificus infection and enhanced immune-related gene expressions
Pan, C.Y., Huang, T.C., Wang, Y.D., Yeh, Y.C., Hui, C.F., and Chen, J.Y.
Date: 2012
Source: Fish & shellfish immunology   32(6): 947-957 (Journal)
Registered Authors: Chen, Jyh-Yih
Keywords: Epinecidin-1, immune response, Danio rerio, Epinephelus coioides, Vibrio vulnificus
MeSH Terms:
  • Adjuvants, Immunologic/administration & dosage*
  • Administration, Oral
  • Animals
  • Antimicrobial Cationic Peptides/administration & dosage*
  • Antimicrobial Cationic Peptides/genetics
  • Antimicrobial Cationic Peptides/immunology
  • Escherichia coli/genetics
  • Fish Diseases/immunology
  • Fish Diseases/prevention & control*
  • Fish Proteins/administration & dosage*
  • Fish Proteins/genetics
  • Fish Proteins/immunology
  • Gene Expression Regulation/drug effects*
  • Perciformes/genetics
  • Perciformes/immunology*
  • Recombinant Proteins/administration & dosage
  • Recombinant Proteins/genetics
  • Recombinant Proteins/immunology
  • Vibrio Infections/prevention & control
  • Vibrio Infections/veterinary*
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed: 22554570 Full text @ Fish Shellfish Immunol.
ABSTRACT

Immunostimulatory effects of the oral administration of the recombinant epinecidin-1 protein from BL21 Escherichia coli (containing the pET28a-epinecidin-1-dsRed plasmid) were studied in grouper (Epinephelus coioides) and zebrafish (Danio rerio). For this purpose, fish were fed diets for 30 days containing the recombinant epinecidin-1 protein from BL21 E. coli (containing the pET28a-epinecidin-1-dsRed plasmid) at different bacterial numbers (104, 106, 108, and 1010 colony-forming units (cfu) of BL21 E. coli in 50 ml of LB medium) mixed with 50 g of eel powder as fodder. After 30 days of feeding, immune-related gene expressions for bacterial-infection responses and disease resistance against Vibrio vulnificus (204) were determined. The V. vulnificus (204) injected into the fish abdominal cavity mimicked gram-negative bacterial infections in culture ponds. Experimental results assessed whether the recombinant epinecidin-1 protein from BL21 E. coli (containing the pET28a-epinecidin-1-dsRed plasmid) has up- (or down-) regulation immune-related genes expression. Results indicated that the recombinant epinecidin-1 protein from BL21 E. coli administered as a feed supplement significantly enhanced expressions several immune-related genes such as tumor necrosis factor (TNF)-1 in grouper and Toll-like receptor (TLR)4, interleukin (IL)-1β, nitric oxide synthase (NOS)2, and nuclear factor (NF)-κB in zebrafish. After being challenged with V. vulnificus (204) for 24, 48, 72, or 96 h, the percentage mortality was significantly reduced in treated fish, which indicated that the recombinant epinecidin-1 protein from BL21 E. coli administered as a feed supplement could bring about downregulation of TNF-1 expression and functioned like an antagonist for binding TLR4, which reduced the signal transduction pathway for inhibiting TNF and IL-1β expressions while reducing binding of the transcription factor, NF-κB, to TNF and the IL-1β promoter region. The experimental results indicated that dietary intake of the recombinant epinecidin-1 protein from BL21 E. coli modulated immune-related gene expressions and disease resistance of grouper and zebrafish after a V. vulnificus (204) infection.

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