PUBLICATION

Chemokine Signaling Directs Trunk Lymphatic Network Formation along the Preexisting Blood Vasculature

Authors
Cha, Y.R., Fujita, M., Butler, M., Isogai, S., Kochhan, E., Siekmann, A.F., and Weinstein, B.M.
ID
ZDB-PUB-120424-12
Date
2012
Source
Developmental Cell   22(4): 824-836 (Journal)
Registered Authors
Butler, Matthew, Cha, Young, Fujita, Misato, Isogai, Sumio, Siekmann, Arndt Friedrich, Weinstein, Brant M.
Keywords
none
MeSH Terms
  • Animals
  • Cell Communication
  • Cells, Cultured
  • Chemokine CXCL12/genetics
  • Chemokine CXCL12/metabolism*
  • Chemokines, CXC/genetics
  • Chemokines, CXC/metabolism*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Lymphatic System/physiology*
  • RNA Probes
  • Receptors, CXCR4/antagonists & inhibitors
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/metabolism*
  • Recombination, Genetic
  • Signal Transduction*
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
22516200 Full text @ Dev. Cell
Abstract

The lymphatic system is crucial for fluid homeostasis, immune responses, and numerous pathological processes. However, the molecular mechanisms responsible for establishing the anatomical form of the lymphatic vascular network remain largely unknown. Here, we show that chemokine signaling provides critical guidance cues directing early trunk lymphatic network assembly and patterning. The chemokine receptors Cxcr4a and Cxcr4b are expressed in lymphatic endothelium, whereas chemokine ligands Cxcl12a and Cxcl12b are expressed in adjacent tissues along which the developing lymphatics align. Loss- and gain-of-function studies in zebrafish demonstrate that chemokine signaling orchestrates the stepwise assembly of the trunk lymphatic network. In addition to providing evidence for a lymphatic vascular guidance mechanism, these results also suggest a molecular basis for the anatomical coalignment of lymphatic and blood vessels.

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Errata and Notes