PUBLICATION

Attenuated BMP1 Function Compromises Osteogenesis, Leading to Bone Fragility in Humans and Zebrafish

Authors
Asharani, P.V., Keupp, K., Semler, O., Wang, W., Li, Y., Thiele, H., Yigit, G., Pohl, E., Becker, J., Frommolt, P., Sonntag, C., Altmüller, J., Zimmermann, K., Greenspan, D.S., Akarsu, N.A., Netzer, C., Schönau, E., Wirth, R., Hammerschmidt, M., Nürnberg, P., Wollnik, B., and Carney, T.J.
ID
ZDB-PUB-120410-3
Date
2012
Source
American journal of human genetics   90(4): 661-674 (Journal)
Registered Authors
Carney, Tom, Greenspan, Daniel S., Hammerschmidt, Matthias, P.V., Asharani, Sonntag, Carmen
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Bone Density Conservation Agents/therapeutic use
  • Bone Morphogenetic Protein 1/genetics
  • Bone Morphogenetic Protein 1/metabolism
  • Bone Morphogenetic Protein 1/physiology*
  • Bone and Bones/metabolism
  • Cell Differentiation
  • Child, Preschool
  • Collagen/biosynthesis
  • Diphosphonates/therapeutic use
  • Exome
  • Female
  • Fractures, Bone/drug therapy
  • Fractures, Bone/prevention & control
  • Genetic Loci
  • Heat-Shock Proteins
  • Humans
  • Male
  • Molecular Sequence Data
  • Mutation
  • Osteoblasts/drug effects
  • Osteoblasts/physiology
  • Osteogenesis/drug effects
  • Osteogenesis/genetics*
  • Osteogenesis/physiology*
  • Peptide Fragments
  • Protein Processing, Post-Translational
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
22482805 Full text @ Am. J. Hum. Genet.
Abstract

Bone morphogenetic protein 1 (BMP1) is an astacin metalloprotease with important cellular functions and diverse substrates, including extracellular-matrix proteins and antagonists of some TGFβ superfamily members. Combining whole-exome sequencing and filtering for homozygous stretches of identified variants, we found a homozygous causative BMP1 mutation, c.34G>C, in a consanguineous family affected by increased bone mineral density and multiple recurrent fractures. The mutation is located within the BMP1 signal peptide and leads to impaired secretion and an alteration in posttranslational modification. We also characterize a zebrafish bone mutant harboring lesions in bmp1a, demonstrating conservation of BMP1 function in osteogenesis across species. Genetic, biochemical, and histological analyses of this mutant and a comparison to a second, similar locus reveal that Bmp1a is critically required for mature-collagen generation, downstream of osteoblast maturation, in bone. We thus define the molecular and cellular bases of BMP1-dependent osteogenesis and show the importance of this protein for bone formation and stability.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping