Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish
- Authors
- Kuscha, V., Frazer, S.L., Dias, T.B., Hibi, M., Becker, T., and Becker, C.G.
- ID
- ZDB-PUB-120409-3
- Date
- 2012
- Source
- The Journal of comparative neurology 520(16): 3604-3616 (Journal)
- Registered Authors
- Becker, Catherina G., Dias, Tatyana, Frazer, Sarah, Hibi, Masahiko, Kuscha, Veronika
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axotomy
- Immunohistochemistry
- In Situ Hybridization
- Interneurons/cytology*
- Interneurons/metabolism
- Nerve Regeneration/physiology*
- Neural Stem Cells/cytology*
- Neural Stem Cells/metabolism
- Spinal Cord Injuries/pathology*
- Zebrafish/physiology*
- PubMed
- 22473852 Full text @ J. Comp. Neurol.
In contrast to mammals, adult zebrafish regenerate neurons in the the lesioned spinal cord. For example, motor neurons are generated from an olig2 expressing population of pMN-like ependymo-radial glial cells in a ventro-lateral position at the central canal. However, the extent of neuronal regeneration is unclear. Here we show, using a transgenic fish in which V2 interneurons are labeled by green fluorescent protein (GFP) under the control of the vsx1 promoter, that after a complete spinal cord transection, large numbers of V2 interneurons are generated in the vicinity of the lesion site. Tg(vsx1:GFP)+ cells are not present in the unlesioned spinal cord and label with the proliferation marker BrdU after a lesion. Some medio-laterally elongated Tg(vsx1:GFP)+ cells contact the central canal in a medial position. These cells likely arise from a p2-like domain of ependymo-radial glial progenitor cells, indicated by co-expression of Pax6 and Nkx6.1, but not DsRed driven by the olig2 promoter in these cells. We also present evidence that Pax2+ interneurons are newly generated after a spinal lesion, whereas the generation rate for a dorsal population of parvalbuminergic interneurons is comparatively low. Our results identify the regenerative potential of different interneuron types for the first time and support a model in which different progenitor cell domains in distinct dorso-ventral positions around the central canal are activated by a lesion to give rise to diverse neuronal cell types in the adult zebrafish spinal cord.