PUBLICATION

Assessing teratogenic changes in a zebrafish model of fetal alcohol exposure

Authors
Loucks, E., and Ahlgren, S.
ID
ZDB-PUB-120329-8
Date
2012
Source
Journal of visualized experiments : JoVE   (61): (Journal)
Registered Authors
Ahlgren, Sara, Loucks, Evyn
Keywords
none
MeSH Terms
  • Abnormalities, Drug-Induced/etiology*
  • Alcohol-Induced Disorders
  • Animals
  • Disease Models, Animal*
  • Embryo, Nonmammalian/abnormalities
  • Embryo, Nonmammalian/drug effects
  • Ethanol/toxicity*
  • Female
  • Male
  • Zebrafish/abnormalities*
  • Zebrafish/embryology
PubMed
22453686 Full text @ J. Vis. Exp.
Abstract

Fetal alcohol syndrome (FAS) is a severe manifestation of embryonic exposure to ethanol. It presents with characteristic defects to the face and organs, including mental retardation due to disordered and damaged brain development. Fetal alcohol spectrum disorder (FASD) is a term used to cover a continuum of birth defects that occur due to maternal alcohol consumption, and occurs in approximately 4% of children born in the United States. With 50% of child-bearing age women reporting consumption of alcohol, and half of all pregnancies being unplanned, unintentional exposure is a continuing issue2. In order to best understand the damage produced by ethanol, plus produce a model with which to test potential interventions, we developed a model of developmental ethanol exposure using the zebrafish embryo. Zebrafish are ideal for this kind of teratogen study3-8. Each pair lays hundreds of eggs, which can then be collected without harming the adult fish. The zebrafish embryo is transparent and can be readily imaged with any number of stains. Analysis of these embryos after exposure to ethanol at different doses and times of duration and application shows that the gross developmental defects produced by ethanol are consistent with the human birth defect. Described here are the basic techniques used to study and manipulate the zebrafish FAS model.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes