ZFIN ID: ZDB-PUB-120316-5
5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development
Almeida, R.D., Loose, M., Sottile, V., Matsa, E., Denning, C., Young, L., Johnson, A.D., Gering, M., and Ruzov, A.
Date: 2012
Source: Epigenetics   7(4): 383-389 (Journal)
Registered Authors: Gering, Martin
Keywords: none
MeSH Terms:
  • 5-Methylcytosine/metabolism
  • Animals
  • Blastocyst/cytology
  • Blastocyst/metabolism
  • Cell Differentiation
  • Chick Embryo
  • Culture Media, Conditioned
  • Cytosine/analogs & derivatives*
  • Cytosine/metabolism
  • DNA Methylation
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Female
  • Gene Expression Regulation, Developmental*
  • Immunohistochemistry
  • Male
  • Mice
  • Organogenesis
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • Time Factors
  • Vertebrates/embryology*
  • Vertebrates/genetics
  • Vertebrates/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 22419071 Full text @ Epigenetics

5-hydroxymethyl-cytosine (5-hmC) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (ESC) derived from mammalian blastocysts. Recent observations imply that both 5-hmC and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmC, may play an important role in self renewal and differentiation of ESCs. Here we assessed the distribution of 5-hmC in zebrafish and chick embryos and found that, unlike in mammals, 5-hmC is immunochemically undetectable in these systems before the onset of organogenesis. In addition, Tet1/2/3 transcripts are either low or undetectable at corresponding stages of zebrafish development. However, 5-hmC is enriched in later zebrafish and chick embryos and exhibits tissue-specific distribution in adult zebrafish. Our findings show that 5-hmC enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmC in its regulation.