PUBLICATION

Characterization and management of asymptomatic mycobacterium infections at the zebrafish international resource center

Authors
Murray, K.N., Bauer, J., Tallen, A., Matthews, J.L., Westerfield, M., and Varga, Z.M.
ID
ZDB-PUB-120216-5
Date
2011
Source
Journal of the American Association for Laboratory Animal Science : JAALAS   50(5): 675-679 (Journal)
Registered Authors
Bauer, Justin, Matthews, Jennifer, Murray, Katy, Varga, Zoltan M., Westerfield, Monte
Keywords
none
MeSH Terms
  • Animal Husbandry/methods
  • Animals
  • Animals, Laboratory*
  • Asymptomatic Diseases/epidemiology*
  • Fish Diseases/epidemiology*
  • Fish Diseases/microbiology*
  • Laboratory Animal Science/methods
  • Mycobacterium Infections/epidemiology
  • Mycobacterium Infections/veterinary*
  • Mycobacterium chelonae*
  • Prevalence
  • Sanitation/methods
  • Species Specificity
  • Zebrafish*
PubMed
22330714
Abstract

The Zebrafish International Resource Center (ZIRC) supplies wildtype, mutant, and transgenic zebrafish (Danio rerio) to the international research community. In 2005, the ZIRC halted shipment of adult Tübingen (TU) zebrafish, a popular wildtype line, after diagnosis of asymptomatic Mycobacterium chelonae infections in a high proportion of the TU stock. Mycobacterium presents a zoonotic risk to fish handlers. In addition, the presence of underlying chronic disease in a model organism is unacceptable. The TU stock was depopulated and replaced by a new import of TU with the intent of reducing disease prevalence. In the current study, we sampled the new population of TU and fish of the AB, Tupfel long-fin (TL), TAB5 and TAB14 (2 AB × TU hybrid lines), and wildtype-in-Kalkutta (WIK) lines for histologic evaluation and acid-fast staining and compared the prevalence of subclinical mycobacteriosis between these lines. Although prevalence in the new TU stock was lower than that of the original TU stock, asymptomatic infections with Mycobacterium remained high (10%) in the new TU stock held in 20-gal tanks. The prevalence was similar (10%) in the TAB5 line compared with other wildtype lines held in similar conditions. Prevalence of infections in TU can be minimized by husbandry adjustments, including tank size, population density, and cleaning method. Application of these findings has allowed us to decrease mycobacteriosis in TU zebrafish and resume shipment of TU adults to the research community.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes