PUBLICATION

Transmission, Diagnosis, and Recommendations for Control of Pseudoloma neurophilia Infections in Laboratory Zebrafish (Danio rerio) Facilities

Authors
Murray, K.N., Dreska, M., Nasiadka, A., Rinne, M., Matthews, J.L., Carmichael, C., Bauer, J., Varga, Z.M., and Westerfield, M.
ID
ZDB-PUB-120216-3
Date
2011
Source
Comparative medicine   61(4): 322-329 (Journal)
Registered Authors
Bauer, Justin, Carmichael, Carrie, Matthews, Jennifer, Murray, Katy, Nasiadka, Andrzej, Varga, Zoltan M., Westerfield, Monte
Keywords
none
MeSH Terms
  • Animal Husbandry/methods*
  • Animals
  • Animals, Laboratory*
  • DNA Primers/genetics
  • Fish Diseases/diagnosis*
  • Fish Diseases/microbiology*
  • Fish Diseases/prevention & control
  • Fish Diseases/transmission
  • Histological Techniques/veterinary
  • Microsporidia/genetics*
  • Microsporidiosis/diagnosis
  • Microsporidiosis/prevention & control
  • Microsporidiosis/transmission
  • Microsporidiosis/veterinary*
  • Polymerase Chain Reaction/methods
  • Polymerase Chain Reaction/veterinary
  • Zebrafish*
PubMed
22330247
Abstract

The microsporidium Pseudoloma neurophilia represents a considerable challenge for laboratory zebrafish (Danio rerio) facilities. In 2010, P. neurophilia infections were diagnosed in zebrafish from 74% of the facilities that submitted fish to the Zebrafish International Resource Center (ZIRC) pathology service, and this organism remains the most commonly diagnosed pathogen in submitted fish. Accordingly, many of the ZIRC pathology service consultations deal with control and prevention of microsporidiosis. Here we describe observations and experiments performed at the ZIRC elucidating aspects of P. neurophilia transmission in zebrafish colonies. We then review current knowledge about P. neurophilia transmission and diagnosis. Considering this information, we present recommendations for control of P. neurophilia in zebrafish facilities.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes