ZFIN ID: ZDB-PUB-120209-6
Caffeine treatment disturbs the angiogenesis of zebrafish embryos
Yeh, C.H., Liao, Y.F., Chang, C.Y., Tsai, J.N., Wang, Y.H., Cheng, C.C., Wen, C.C., and Chen, Y.H.
Date: 2012
Source: Drug and chemical toxicology   35(4): 361-365 (Journal)
Registered Authors: Liao, Yun-Feng
Keywords: angiogenesis, caffeine, fluorescent protein, transgenic fish, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Caffeine/administration & dosage
  • Caffeine/toxicity*
  • Dose-Response Relationship, Drug
  • Down-Regulation/drug effects
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Endothelium, Vascular/drug effects*
  • Endothelium, Vascular/metabolism
  • Green Fluorescent Proteins/metabolism
  • Neovascularization, Physiologic/drug effects*
  • Nerve Growth Factors/drug effects
  • Nerve Growth Factors/genetics
  • Real-Time Polymerase Chain Reaction
  • Teratogens/toxicity
  • Up-Regulation/drug effects
  • Zebrafish/embryology
  • Zebrafish Proteins/drug effects*
  • Zebrafish Proteins/genetics
PubMed: 22313413 Full text @ Drug Chem. Toxicol.

Caffeine is a widely consumed substance that occurs in numerous dietary sources, but teratogenic effects of caffeine intake during embryonic development are still not clear. In the present study, we used the zebrafish as a model to assess caffeine-induced toxicity on embryonic vascular development. A green fluorescent vascular endothelium transgenic line, Tg(fli1:egfp), was utilized for the sensitive detection of vascular development, including vasculo- and angiogenesis. Caffeine-treated embryos showed no defects in vasculogenesis, but revealed dose-dependent (250–350 ppm) developmental defects in intersegmental vessels, dorsal longitudinal anastomotic vessels, and subintestinal vein sprouting. Further, real-time polymerase chain reaction analysis of caffeine-treated embryos showed an upregulation of nrp1a along with a downregulation of sema3aa and sema3c. In conclusion, caffeine treatment induces defects of angiogenesis in zebrafish embryos.