PUBLICATION
High-Contrast Reversible Fluorescence Photoswitching of Dye-Crosslinked Dendritic Nanoclusters in Living Vertebrates
- Authors
- Kim, Y., Jung, H.Y., Choe, Y.H., Lee, C., Ko, S.K., Koun, S., Choi, Y., Chung, B.H., Park, B.C., Huh, T.L., Shin, I., and Kim, E.
- ID
- ZDB-PUB-120207-15
- Date
- 2012
- Source
- Angewandte Chemie (International ed. in English) 51(12): 2878-2882 (Journal)
- Registered Authors
- Huh, Tae-Lin, Koun, Soonil
- Keywords
- dendrimer, fluorescence, FRET, photoswitch, zebrafish
- MeSH Terms
-
- Animals
- Carbocyanines/chemistry
- Dendrimers/chemistry*
- Fluorescence Resonance Energy Transfer
- Fluorescent Dyes/chemistry*
- HeLa Cells
- Humans
- Microscopy, Fluorescence
- Nanoparticles/chemistry*
- Ultraviolet Rays
- Zebrafish
- PubMed
- 22307986 Full text @ Angew. Chem. Int. Ed. Engl.
Citation
Kim, Y., Jung, H.Y., Choe, Y.H., Lee, C., Ko, S.K., Koun, S., Choi, Y., Chung, B.H., Park, B.C., Huh, T.L., Shin, I., and Kim, E. (2012) High-Contrast Reversible Fluorescence Photoswitching of Dye-Crosslinked Dendritic Nanoclusters in Living Vertebrates. Angewandte Chemie (International ed. in English). 51(12):2878-2882.
Abstract
Dendrimers crosslinked with a photochromic diarylethene (blue in picture) were labeled with the fluorescent dye Cy3 (red) for reversible fluorescence photoswitching. If the obtained nanoclusters are irradiated with UV light, the Cy3 fluorescence is quenched by the diarylethene in its ring-closed form, whereas upon irradiation with visible light, the nanoclusters show fluorescence. High-contrast fluorescence imaging was achieved inside a living zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping