ZFIN ID: ZDB-PUB-120207-15
High-Contrast Reversible Fluorescence Photoswitching of Dye-Crosslinked Dendritic Nanoclusters in Living Vertebrates
Kim, Y., Jung, H.Y., Choe, Y.H., Lee, C., Ko, S.K., Koun, S., Choi, Y., Chung, B.H., Park, B.C., Huh, T.L., Shin, I., and Kim, E.
Date: 2012
Source: Angewandte Chemie (International ed. in English)   51(12): 2878-2882 (Journal)
Registered Authors: Huh, Tae-Lin, Koun, Soonil
Keywords: dendrimer, fluorescence, FRET, photoswitch, zebrafish
MeSH Terms:
  • Animals
  • Carbocyanines/chemistry
  • Dendrimers/chemistry*
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Humans
  • Microscopy, Fluorescence
  • Nanoparticles/chemistry*
  • Ultraviolet Rays
  • Zebrafish
PubMed: 22307986 Full text @ Angew. Chem. Int. Ed. Engl.

Dendrimers crosslinked with a photochromic diarylethene (blue in picture) were labeled with the fluorescent dye Cy3 (red) for reversible fluorescence photoswitching. If the obtained nanoclusters are irradiated with UV light, the Cy3 fluorescence is quenched by the diarylethene in its ring-closed form, whereas upon irradiation with visible light, the nanoclusters show fluorescence. High-contrast fluorescence imaging was achieved inside a living zebrafish.