PUBLICATION
Separation of young and mature thrombocytes by a novel immuno-selection method
- Authors
- Kulkarni, V., Kim, S., Zafreen, L., and Jagadeeswaran, P.
- ID
- ZDB-PUB-120203-3
- Date
- 2012
- Source
- Blood cells, molecules & diseases 48(3): 183-187 (Journal)
- Registered Authors
- Jagadeeswaran, Pudur, Kim, Seongcheol
- Keywords
- immuno-fluorescence, mature thrombocyte, young thrombocyte, streptavidin magnetic beads, Dil-C18, zebrafish
- MeSH Terms
-
- Animals
- Antibodies, Monoclonal/immunology
- Antibodies, Monoclonal/metabolism
- Blood Platelets/chemistry
- Blood Platelets/metabolism*
- Immunomagnetic Separation/methods*
- Methylamines/chemistry
- Methylamines/immunology
- Methylamines/metabolism
- Platelet Glycoprotein GPIb-IX Complex/immunology
- RNA/isolation & purification
- Staining and Labeling
- Zebrafish/metabolism
- PubMed
- 22297253 Full text @ Blood Cells Mol. Dis.
Citation
Kulkarni, V., Kim, S., Zafreen, L., and Jagadeeswaran, P. (2012) Separation of young and mature thrombocytes by a novel immuno-selection method. Blood cells, molecules & diseases. 48(3):183-187.
Abstract
Our earlier studies on the structural and functional properties of zebrafish thrombocytes have shown that they have many similarities to mammalian platelets. We have also shown that zebrafish have both young and mature thrombocytes as do mammalian platelets. In addition, we have distinguished young thrombocytes from mature thrombocytes microscopically using lipophilic DiI-C18, and have shown that young thrombocytes have higher GPIIb receptor levels. However, at present, there is no immunoselection method to separate young thrombocytes from mature thrombocytes in order to study differences among them, such as mRNA expression levels of thrombocyte specific genes. We developed a novel technique employing specific biotinylated anti-Cy3 antibody against the chromophore of DiI-C18 and using streptavidin magnetic beads to separate young thrombocytes from mature thrombocytes. Our technique separates and differentiates young and mature thrombocytes from whole blood. This method is specific and is effective with small amounts of blood.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping