The transcriptional activator ZNF143 is essential for normal development in zebrafish
- Authors
- Halbig, K.M., Lekven, A.C., and Kunkel, G.R.
- ID
- ZDB-PUB-120125-42
- Date
- 2012
- Source
- BMC Molecular Biology 13(1): 3 (Journal)
- Registered Authors
- Lekven, Arne
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Cardiac Myosins/genetics
- Cardiac Myosins/metabolism
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- GATA1 Transcription Factor/genetics
- GATA1 Transcription Factor/metabolism
- Morpholinos
- Myosin Light Chains/genetics
- Myosin Light Chains/metabolism
- PAX2 Transcription Factor/genetics
- PAX2 Transcription Factor/metabolism
- Promoter Regions, Genetic
- Trans-Activators/antagonists & inhibitors
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Zebrafish/embryology
- Zebrafish/metabolism*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 22268977 Full text @ BMC Mol. Biol.
Background
ZNF143 is a sequence-specific DNA-binding protein that stimulates transcription of both small RNA genes by RNA polymerase II or III, or protein-coding genes by RNA polymerase II, using separable activating domains. We describe phenotypic effects following knockdown of this protein in developing Danio rerio (zebrafish) embryos by injection of morpholino antisense oligonucleotides that target znf143 mRNA.
Results
The loss of function phenotype is pleiotropic and includes a broad array of abnormalities including defects in heart, blood, ear and midbrain hindbrain boundary. Defects are rescued by coinjection of synthetic mRNA encoding full-length ZNF143 protein, but not by protein lacking the amino-terminal activation domains. Accordingly, expression of several marker genes is affected following knockdown, including GATA-binding protein 1 (gata1), cardiac myosin light chain 2 (cmlc2) and paired box gene 2a (pax2a). The zebrafish pax2a gene proximal promoter contains two binding sites for ZNF143, and reporter gene transcription driven by this promoter in transfected cells is activated by this protein.
Conclusions
Normal development of zebrafish embryos requires ZNF143. Furthermore, the pax2a gene is probably one example of many protein-coding gene targets of ZNF143 during zebrafish development.