PUBLICATION
Animated bird silhouette above the tank: Acute alcohol diminishes fear responses in zebrafish
- Authors
- Luca, R.M., and Gerlai, R.
- ID
- ZDB-PUB-120125-38
- Date
- 2012
- Source
- Behavioural brain research 229(1): 194-201 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- alcoholism, anxiety, EtOH, fear, zebrafish
- MeSH Terms
-
- Analysis of Variance
- Animals
- Anxiety/drug therapy*
- Anxiety/etiology
- Behavior, Animal/drug effects
- Birds
- Central Nervous System Depressants/pharmacology*
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Ethanol/pharmacology*
- Fear/drug effects*
- Freezing Reaction, Cataleptic/drug effects
- Motor Activity/drug effects
- Photic Stimulation/adverse effects
- Visual Perception/drug effects
- Zebrafish/physiology*
- PubMed
- 22266470 Full text @ Behav. Brain Res.
Citation
Luca, R.M., and Gerlai, R. (2012) Animated bird silhouette above the tank: Acute alcohol diminishes fear responses in zebrafish. Behavioural brain research. 229(1):194-201.
Abstract
Alcohol dependence and alcohol abuse represent major unmet medical needs. The zebrafish is considered to be a promising vertebrate species with which the effects of alcohol on brain function and behavior and the mechanisms underlying these effects may be studied. Alcohol is known to induce alterations in motor function as well as fear and anxiety. Here we present a recently developed fear paradigm in which we employ an animated (moving) image of a bird silhouette. We measure the effect of acute alcohol administration (dose range employed: 0.00–0.75 vol/vol percentage, bath exposure for 60 min) on the behavioral responses of zebrafish. We test these responses during a pre-stimulus, stimulus and post-stimulus period of the task using both a video-tracking and an observation based quantification method. The fear inducing stimulus was found to decrease the distance of the zebrafish from the bottom of the tank, to increase number of erratic movements, and to increase the number of jumps in alcohol exposed fish (versus control fish). Alcohol attenuated these fear responses in a dose dependent manner. In addition, alcohol decreased general activity at the highest dose, an effect that was independent of the presentation of the stimulus. We discuss the similarities and differences between observation and video-tracking based results and conclude that fear paradigms will be useful in revealing alcohol induced functional changes in the brain of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping