ZFIN ID: ZDB-PUB-120111-22
Visualizing vascular networks in zebrafish: an introduction to microangiography
Schmitt, C.E., Holland, M.B., and Jin, S.W.
Date: 2012
Source: Methods in molecular biology (Clifton, N.J.)   843: 59-67 (Chapter)
Registered Authors: Jin, Suk-Won
Keywords: microangiography, zebrafish, vascular lumen, cardiovascular development
MeSH Terms:
  • Angiography/instrumentation
  • Angiography/methods*
  • Animals
  • Blood Vessels/anatomy & histology
  • Blood Vessels/embryology*
  • Microinjections
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
PubMed: 22222521 Full text @ Meth. Mol. Biol.
ABSTRACT
Visualizing the circulatory pattern in developing embryos becomes an essential technique for the field of cardiovascular biology. In the zebrafish model system, there are currently several techniques available to visualize the circulatory pattern. Microangiography is a simple technique in which a fluorescent dye is injected directly into the Sinus Venosus and/or the Posterior Cardinal Vein, allowing for the rapid labeling and easy detection of patent vessels. Here, we compare microangiography to other vascular labeling techniques, describe the benefits and potential applications of microangiography, and give step by step instructions for microangiography.
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