PUBLICATION
Interordinal Chimera Formation between Medaka and Zebrafish for Analyzing Stem Cell Differentiation
- Authors
- Hong, N., Chen, S.L., Ge, R., Song, J., Yi, M., and Hong, Y.
- ID
- ZDB-PUB-120110-1
- Date
- 2012
- Source
- Stem cells and development 21(12): 2333-2341 (Journal)
- Registered Authors
- Ge, Ruowen, Yi, Meisheng
- Keywords
- none
- MeSH Terms
-
- Animals
- Antigens, Differentiation/genetics
- Antigens, Differentiation/metabolism
- Blastomeres/cytology
- Cell Differentiation*
- Cell Survival
- Chimera/embryology
- Chimera/genetics*
- Embryo Culture Techniques
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/transplantation
- Embryonic Development
- Embryonic Stem Cells/metabolism
- Embryonic Stem Cells/physiology*
- Embryonic Stem Cells/transplantation
- Gene Expression
- Oryzias/embryology
- Oryzias/genetics*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 22204449 Full text @ Stem Cells Dev.
Citation
Hong, N., Chen, S.L., Ge, R., Song, J., Yi, M., and Hong, Y. (2012) Interordinal Chimera Formation between Medaka and Zebrafish for Analyzing Stem Cell Differentiation. Stem cells and development. 21(12):2333-2341.
Abstract
Chimera formation is a standard test for pluripotency of stem cells in vivo. Interspecific chimera formation between distantly related organisms offers also an attractive approach for propagating endangered species. Parameters influencing interspecies chimera formation have remained poorly elucidated. Here we report interordinal chimera formation between medaka and zebrafish, which separated approximately 320 million years ago and exhibit a more than 2-fold difference in developmental speed. We show that, upon transplantation into zebrafish blastulae, both non-cultivated blastomeres and long-term cultivated ES cells of medaka adopted the zebrafish developmental program and differentiated into physiologically functional cell types including pigment cells, blood cells and myocardiocytes. We also show that medaka ES cells express differentiation gene markers during chimeric embryogenesis. Therefore, the evolutionary distance and different embryogenesis speeds do not produce donor-host incompatibility to compromise chimera formation between medaka and zebrafish, and molecular markers are valuable for analyzing lineage commitment and cell differentiation in interspecific chimeric embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping