PUBLICATION
BAPTI and BAPTISM Birthdating of Neurons in Zebrafish
- Authors
- Pan, Y.A., Caron, S.J., and Schier, A.F.
- ID
- ZDB-PUB-120106-20
- Date
- 2012
- Source
- Cold Spring Harbor protocols 2012(1): pdb.prot067520 (Journal)
- Registered Authors
- Caron, Sophie, Pan, Y. Albert, Schier, Alexander
- Keywords
- none
- MeSH Terms
-
- Animals
- Developmental Biology/methods*
- Fluorescent Dyes/chemistry
- Fluorescent Dyes/metabolism*
- Neurons/physiology*
- Staining and Labeling/methods*
- Trigeminal Nerve/cytology
- Trigeminal Nerve/growth & development*
- Zebrafish/growth & development*
- PubMed
- 22194263 Full text @ Cold Spring Harb. Protoc.
Citation
Pan, Y.A., Caron, S.J., and Schier, A.F. (2012) BAPTI and BAPTISM Birthdating of Neurons in Zebrafish. Cold Spring Harbor protocols. 2012(1):pdb.prot067520.
Abstract
This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish. The methods used are birthdating analysis by photoconverted fluorescent protein tracing in vivo (BAPTI) and BAPTI combined with subpopulation markers (BAPTISM). Because Kaede can be converted from green to red fluorescence at any developmental time point, it serves as a temporal landmark for cell birth. When it is used in combination with subpopulation markers, the eventual fate of a cell can be correlated with its birthdate. We describe how we used this method to study the development of trigeminal sensory neurons and discuss how the technique can be extended to the study of other organs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping