PUBLICATION

Multiple influences of blood flow on cardiomyocyte hypertrophy in the embryonic zebrafish heart

Authors
Lin, Y.F., Swinburne, I., and Yelon, D.
ID
ZDB-PUB-120106-18
Date
2012
Source
Developmental Biology   362(2): 242-253 (Journal)
Registered Authors
Swinburne, Ian, Yelon, Deborah
Keywords
cardiomyocyte, cardiac chamber formation, hypertrophy, myofibril, α-Actinin, blood flow, weak atrium
MeSH Terms
  • Luminescent Proteins
  • Fluorescent Antibody Technique
  • Myocytes, Cardiac/pathology*
  • Myocytes, Cardiac/physiology*
  • Hemodynamics
  • Heart Ventricles/embryology*
  • DNA Primers/genetics
  • Models, Biological*
  • Regional Blood Flow/physiology*
  • Animals
  • Zebrafish/embryology*
  • Morphogenesis/physiology*
  • Cell Enlargement
(all 13)
PubMed
22192888 Full text @ Dev. Biol.
Abstract

Cardiomyocyte hypertrophy is a complex cellular behavior involving coordination of cell size expansion and myofibril content increase. Here, we investigate the contribution of cardiomyocyte hypertrophy to cardiac chamber emergence, the process during which the primitive heart tube transforms into morphologically distinct chambers and increases its contractile strength. Focusing on the emergence of the zebrafish ventricle, we observed trends toward increased cell surface area and myofibril content. To examine the extent to which these trends reflect coordinated hypertrophy of individual ventricular cardiomyocytes, we developed a method for tracking cell surface area changes and myofibril dynamics in live embryos. Our data reveal a previously unappreciated heterogeneity of ventricular cardiomyocyte behavior during chamber emergence: although cardiomyocyte hypertrophy was prevalent, many cells did not increase their surface area or myofibril content during the observed timeframe. Despite the heterogeneity of cell behavior, we often found hypertrophic cells neighboring each other. Next, we examined the impact of blood flow on the regulation of cardiomyocyte behavior during this phase of development. When blood flow through the ventricle was reduced, cell surface area expansion and myofibril content increase were both dampened, and the behavior of neighboring cells did not seem coordinated. Together, our studies suggest a model in which hemodynamic forces have multiple influences on cardiac chamber emergence: promoting both cardiomyocyte enlargement and myofibril maturation, enhancing the extent of cardiomyocyte hypertrophy, and facilitating the coordination of neighboring cell behaviors.

Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m58
    Small Deletion
    sd10TgTransgenic Insertion
      sd11TgTransgenic Insertion
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        myh6MO1-myh6MRPHLNO
        1 - 1 of 1
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        Fish
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        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        mKate2EFGmKate2
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        Mapping
        No data available