PUBLICATION

Nuclear progesterone receptors are up-regulated by estrogens in neurons and radial glial progenitors in the brain of zebrafish

Authors
Diotel, N., Servili, A., Gueguen, M.M., Mironov, S., Pellegrini, E., Vaillant, C., Zhu, Y., Kah, O., and Anglade, I.
ID
ZDB-PUB-111206-12
Date
2011
Source
PLoS One   6(11): e28375 (Journal)
Registered Authors
Diotel, Nicolas, Kah, Olivier, Zhu, Yong
Keywords
Progesterone, Zebrafish, Larvae, Arcuate nucleus, Neurons, Cell staining, Mammals, Estrogens
MeSH Terms
  • Neuroglia/cytology
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Gene Expression Profiling
  • Up-Regulation/drug effects
  • Up-Regulation/genetics*
  • Animals
  • Preoptic Area/cytology
  • Preoptic Area/drug effects
  • Preoptic Area/metabolism
  • Receptors, Progesterone/genetics*
  • Receptors, Progesterone/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Stem Cells/cytology
  • Stem Cells/drug effects
  • Stem Cells/metabolism*
  • Brain/cytology
  • Brain/growth & development
  • Brain/metabolism*
  • Estrogens/pharmacology*
  • Protein Transport/drug effects
  • Neurons/cytology
  • Neurons/drug effects
  • Neurons/metabolism*
  • Estradiol/pharmacology
  • Gene Expression Regulation, Developmental/drug effects
(all 28)
PubMed
22140581 Full text @ PLoS One
Abstract

In rodents, there is increasing evidence that nuclear progesterone receptors are transiently expressed in many regions of the developing brain, notably outside the hypothalamus. This suggests that progesterone and/or its metabolites could be involved in functions not related to reproduction, particularly in neurodevelopment. In this context, the adult fish brain is of particular interest, as it exhibits constant growth and high neurogenic activity that is supported by radial glia progenitors. However, although synthesis of neuroprogestagens has been documented recently in the brain of zebrafish, information on the presence of progesterone receptors is very limited. In zebrafish, a single nuclear progesterone receptor (pgr) has been cloned and characterized. Here, we demonstrate that this pgr is widely distributed in all regions of the zebrafish brain. Interestingly, we show that Pgr is strongly expressed in radial glial cells and more weakly in neurons. Finally, we present evidence, based on quantitative PCR and immunohistochemistry, that nuclear progesterone receptor mRNA and proteins are upregulated by estrogens in the brain of adult zebrafish. These data document for the first time the finding that radial glial cells are preferential targets for peripheral progestagens and/or neuroprogestagens. Given the crucial roles of radial glial cells in adult neurogenesis, the potential effects of progestagens on their activity and the fate of daughter cells require thorough investigation.

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as100TgTransgenic Insertion
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    GFPEFGGFP
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