ZFIN ID: ZDB-PUB-111205-6
Immunocytochemistry to study myogenesis in zebrafish
Bird, N.C., Windner, S.E., and Devoto, S.H.
Date: 2012
Source: Methods in molecular biology (Clifton, N.J.)   798: 153-169 (Chapter)
Registered Authors: Bird, Nathan C., Devoto, Stephen Henri
Keywords: none
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Immunohistochemistry/methods*
  • Muscle Development/physiology*
  • Tissue Fixation/methods
  • Zebrafish/embryology*
  • Zebrafish Proteins/analysis
PubMed: 22130836 Full text @ Meth. Mol. Biol.
During myogenesis, cells gradually transition from mesodermal precursors to myoblasts, myocytes, and then to muscle fibers. The molecular characterization of this process requires the ability to identify each of these cell types and the factors that regulate the transitions between them. The most versatile technique for assaying cell identities in situ is immunocytochemistry, because multiple independent molecular markers of differentiation can be assayed simultaneously. The zebrafish has developed into a popular model for the study of myogenesis, and immunocytochemical techniques have been critical. We have adapted existing protocols to optimize immunocytochemistry in zebrafish, and have tested many antibodies developed against mouse, chick, and frog muscle antigens for their cross-reactivity in zebrafish. Here, we present protocols for whole mount immunocytochemistry on both formaldehyde and Carnoy's fixed embryos as well as on sectioned zebrafish tissue. We include a table of antibodies useful for experiments on the molecular biology of myogenesis in zebrafish.